口蹄疫抗原决定簇融合基因转化拟南芥初步研究.pdf

104 广东农业科学2011年第17期 口蹄疫抗原决定簇融合基因转化拟南芥初步研究 周 岩，薛晓锋，魏琦超，张 洁，游 建 (河南科技学院生命科技学院。河南新乡453003) 摘要：利用根癌农杆菌介导法将携带有O型和A型El蹄疫抗原决定簇融合基因O：。一O。州：。的双元表达载体对拟南芥外植 体进行遗传转化，并探讨了不同侵染时间和菌液浓度对农杆菌遗传转化效率的影响。通过潮霉素筛选，共获得522块抗性愈伤组 织．经再乍培养得到36棵再生苗，对部分再牛植株进行PCR检测，结果表明目的基因已整合到拟南芥基因组中。 关键词：口蹄疫抗原决定簇；拟南芥：根癌农杆菌 中图分类号：Q78 文献标识码：A 文章编号：1004-874X(2011)17-0104-02 onFMDV determinantfused Preliminary antigenic study thaliana genetransformingArabidopsis ZHOU Jian Yan，XUEXiao-feng，WEIQi-chao，ZHANGJie，YOU and Institute and (Collegeof￡咖neeTechnology,HenanofScietweTechnology,Xinxiaag453003，China) ofFMDV WaS Abstract：Theconstruct OandA determinantfused 021一D14-Atransformedintothe carrying types antigenic gene 21 whole of thdiana transformationmethod．Theoftransformation the plantArabidopsisbyAgrobacterium-mediated impact efficiencyby infectiontimeand concentrationwere calluswereobtained onthe Agrobacterium investigated．522hygromyein-resistant byscreening medium antibiotic，and36 were after culture．Confimmionofthe containing regenerativeplantletsacquiredregenerative transgenicplants PCR demonstratedthat hasbeen into ofthetransformed by analysis targetgene integratedgenome plants． words：FMDVdeterminantfused Key antigenic gene；Arabidopsisthdiana；Agrobacteriumtumefacien 口蹄疫(foot—and—mouthdisease．FMD)是一种由口蹄 拟南