针灸内关穴预处理对兔缺血再灌注损伤心肌细胞死亡受体通路Fas%2fFasL蛋白表达的影响.pdfVIP

针灸内关穴预处理对兔缺血再灌注损伤心肌 细胞死亡受体通路Fas/FasL蛋白表达的影响 林海波严洁常小荣刘密 王超 阳晶晶谢文娟 湖南中医药大学针灸推拿学院，长沙410007 摘要：目的：探讨针灸内关穴预处理对兔缺血再灌注损伤心肌细胞死亡受体通路Fas/FasL蛋白表达的影响。 方法：将24只家兔随机分为假手术组、缺血再灌注组、电针组和艾灸组。采用结扎左冠状动脉前降支40min再灌 注60min的方法，建立心肌缺血再灌注损伤模型。电针组和艾灸组分别在针/灸预处理之后48h再行缺血再灌注。应 用免疫组织化学法，以累积光密度为主要指标观察Fas/FasL蛋白表达的水平。结果：缺血再灌注组心肌组织中Fas 蛋白的阳性表达较假手术组增多(P＜0.05)，电针组和艾灸组较缺血再灌注组均显著降低(P＜0.05),且两组间 无显著性差异；缺血再灌注组和艾灸组心肌组织中FasL蛋白的阳性表达较假手术组增多(P＜0.05),电针组较缺 血再灌注组显著降低(P＜0.05)。结论：针灸内关穴预处理能抑制死亡受体通路Fas/FasL蛋白的表达，从而抑制 缺血再灌注后细胞凋亡的发生，由此对心肌细胞发挥延迟相的保护作用。 关键词：针灸；内关穴；心肌；缺血再灌注损伤；Fas/FasL Effectsofpreacupuncture‘Neiguan(PC6)onFas/FasLproteinexpressionindeath receptorpathwayofmyocardialischemiareperfusioninjurycellinrabbits LINHai-boYANJieCHANGXiao-rongLIUMiWANG ChaoYANGJing-jingXIEWen-juan CollegeofAcu-MoxibustionandMassage,HunanUniversityofChineseMedicine,Changsha410007,China Abstract:Objective:Tostudytheeffectofpreacupuncture ‘Neiguan(PC6)onFas/FasLproteinexpressionindeath receptorpathwayofmyocardialischemiareperfusioninjurycellinrabbits.Methods:24rabbitswererandomlydividedintosham operationgroup,ischemiareperfusiongroup,electroacupuncturegroupandmoxihnstiongroup.Theisebemiareperfusionmodels wereestablishedwithligationoftheleftanteriordescendingcoronaryarterylastingfor40minutesandfollowedbyreperfusionfor 60minutes.Theratsinelectroacupuncturegroupandmoxibustiongroupreceivedtheacupunctureofmoxibustiontreatmentfor 48hbeforeisehemiareperfusion.ThentheexpressionofFas/FasLwasmeasuredbyimmanohistochemicaltechnique,withintegral opticaldensityasthemainindextoobservetheFas/FasLproteinexpression.Results:TheFasexpressionwasmoresignificantly intheischemiareperfusiongroupthanthesham-operationgroup(P＜0.05),butsignificantlydecreasedintelectroacupuncture groupandmoxibustiongroup(P＜0.05),andtherewasnoobviousdifferencebetweenthelasttwogroups.Theexpressionof FasLmarkedlyincreasedintheischemiareperfusionandmoxibustiongroupswithcomp