甘蓝自交不亲和信号传导中THL1和SRK相互作用的体外检测.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2008, 34(6): 934−943 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2008.00934 甘蓝自交不亲和信号传导中THL1 和SRK 相互作用的体外检测 高启国1 宋 明1 牛 义1 杨 昆2 朱利泉2,* 王小佳1,* (1 , 400716; 2 , 400716) : SRK THL1 , E1 , RT-PCR THL1 SRK ( SRKE1), THL1 pET43.1-THL1, SRKE1 pET431-SRKE1 pPIC9K-SRKE1 , BL21 GS115 THL1 , THL1 , THL1 SRKE1 , THL1 SRKE1 , THL1 SRK : S-; ; Test of Interaction between THL1 and SRK from Brassica oleracea L. in Self-Incompatibility Signaling Process 1 1 1 2 2,* 1,* GAO Qi-Guo , SONG Ming , NIU Yi , YANG Kun , ZHU Li-Quan , and WANG Xiao-Jia (1 Key Laboratory in Olericulture of Chongqing, Southwest University, Chongqing 400716; 2 Plant Physiology and Biochemistry Laboratory of Southwest University, Chongqing 400716, China) Abstract: The SRK and THL1 from Brassica oleracea L. act probably with each other in self-incompatibility signal process. In present study, with an aim to testify the interaction, the coding sequences of THL1 and SRK kinase domain(named as SRKE1) were amplified from stigma mRNA of Brassica oleracea L., and inserted into the expression vector pET43.1 and yeast expression vec- tor pPIC9K to construct the recombinant plasma pET43.1-THL1, pET43.1-SRKE1, and pPIC9K-SRKE1 . The recombinant protein were expressed in E. coli(BL21) and Pichia pastoris (GS115), respectively. Thioredoxin activity was tested by the ability of THL1 reducing insulin, showing that THL1 had oxidation/reduction activity. A ne