高糖、高脂肪酸饮食对大鼠骨骼肌葡萄糖转运体4mRNA表达的影响.pdfVIP

±垦蕉垦堑鲞查!!!!生筮!!鲞笙!塑里!垫』垡!!!生!!坚!!生!!!!!!!!!!!塑!! ·161· ·分子生物学· 高糖、高脂肪酸饮食对大鼠骨骼肌葡萄糖 转运体4mRNA表达的影响 贾维娜 宋光耀 张咏梅 高宇 周宇 【提要】成年Wistar大鼠随机分为对照组、高糖组、高饱和脂肪酸组、高不饱和脂肪酸组，喂养24周 后，各实验组与对照组相比葡萄糖输注率(GIR)明显减低(P0．01)，大鼠骨骼肌葡萄糖转运体4 (GluT4)mRNA的表达显著降低(Po．01)，其表达与游离脂肪酸呈明显负相关，与GIR呈明显正相关。 高糖、高饱和脂肪酸及高不饱和脂肪酸饮食均可造成大鼠骨骼肌GluT4mRNA的表达降低，这可能是胰 岛素抵抗的原因。 【关键词】葡萄糖转运体4；骨骼肌；饮食；胰岛素抵抗 on of mRNA ofrats Effectsof sucrOSEand aciddietsthe GluT4 inskeletalmuscle high highfatty expression IA a1．HebeiInstituteGeriatrics，HebeiProvin- J Wei_na，SoNG Yong—mei，et of Guang-yao，zHANG cial 050051，China People’sHospital。Shijiazhuang author：SoNG Corresponding Guang-yao，E-mail：sguangyao@sohu．COgtl theeffectof saturatedac试(HSFA)andun- [SummarylToexplore highsucrose(HS)，highfatty high oninsulinresistance(IR)andthe ofGluT4 inskele- saturatedacid(HUFA)diets fatty expressionprotein talmuscle．AdultWistarratsweredevidedinto ofnormalcontrol(NC)，HS，HSFA，HUFA． groups AndIRwasevaluated infusionrate(GIR)of after24weeksofdiettreatment． byglucose clamptechnique ThemRNA ofGluT4wasmeasuredRT—PCR．GIRwas lowerin expression by obviously experimental of in thanthatinNC GluT4mRNAskeletalmusclewas decreased groups group．Theexpression markedly inall withNC．The ofGluT4inskeletalmusclewas experimentalgroupscompared expression negatively withfree well correlatedwithGIR．The ofGluT4 correlated acid(FFA)and fatty positively expression