神经生长因子脂质体大鼠鼻腔给药的药效学研究.pdfVIP

神经生长因子脂质体大鼠鼻腔给药的药效学研究 谢英，杜熠，耿兴超，侯新朴。(北京大学药学院药剂系，北京100083) 摘要：目的对神经生长因子(nerve growth 药相比较。方法鹅膏蕈氨酸(ibotenicacid，IBO)大鼠Meynert核注射制造阿尔茨海默氏病(alzheimer’S 并应用此模型采用同样方法研究NGF静脉给药，NGF和NGF-SSL鼻腔给药后的药效。结果AD模型经鉴定能够应用于药效 神经元免受损伤，动物游泳时间缩短，跳台潜伏期延长，AchE染色积分光密度接近正常大鼠。结论鼻腔给药能够实现药物 给药途径上的脑靶向，药物经脂质体包载后能明显增加其脑摄入，提高药效。 关键词：神经生长因子；脂质体；鼻腔给药；药效 中图分类号：R969．4 文献标识码：A 文章编号：1001—2494(2005)10—0742—05 ontrealmentofAD NGF vianasaladministration Study by liposomes Yi，GENGXing-chao，HOUofPharm,叫，SchoolofPharmaceutical XIEYing，DU Xin—pu+(Department 8e访愕100083，Ch／nal To nasal ABSTRACI：OBJECllVEthe ofNGFandNGF stabilized studyphannacedynamicparameters sterically and with intravenous wasmade nucleus administration NGF Alzheimer’sdisease(AD)model compare inject．METHODS byMeynert inject— ed访thibotenic wasevaluatedtllewatermaze，footshockavoidanceandAchE tests．1he acid(IBO)．and by Histochemistrystaining pharma— be of studied theADmodel．RE吼Ⅱ门暇ADmodelWas to cedynamie NGF(iv)andNGF，NGF-SSL(na)werebyusing appraised parameters of nasal betterthanthatofNGFin· suitablefor the NGF．r11le ofNGFvia administrationwas studyingpharmacologyefficacy pharmacedynamics travenous thatofNGF-SSLvianasaladministrationWasfu血er administrationthe neuronfrom inject，and improved．Nasal protectedcholinergic swimtimeoftheratsWas thefootshockavoidanceWas byIBO，the shorten，and latencyextended，t