产雄孤雌生殖单倍体胚胎干细胞产生活的转基因小鼠.pdfVIP

LETTER doi:10.1038/nature11435 Androgenetic haploid embryonic stem cells produce live transgenic mice 1,2 1,3 1,2 1,2 4 1,2 1,2 4 Wei Li *, Ling Shuai *, Haifeng Wan *, Mingzhu Dong *, Meng Wang , Lisi Sang , Chunjing Feng , Guan-Zheng Luo , 1,3 1,3 1,2 1,2 1,3 2,4 3 1 1 Tianda Li , Xin Li , Libin Wang , Qin-Yuan Zheng , Chao Sheng , Hua-Jun Wu , Zhonghua Liu , Lei Liu , Liu Wang , Xiu-Jie Wang4 , Xiao-Yang Zhao1 Qi Zhou1 Haploids and double haploids are important resources for study- protein (eGFP) from the donor sperms was successfully inherited ing recessive traits and have large impacts on crop breeding1, but and expressed in the derived ahES-cell lines (Fig. 1a). We also natural haploids are rare in animals. Mammalian haploids are established ahES-cell lines carrying an Oct4 (also known as Pou5f1) restricted to germline cells and are occasionally found in tumours promoter-driven eGFP (Oct4–eGFP) reporter, by which eGFP expres- with massive chromosome loss2,3. Recent success in establishing sion was primarily restricted in pluripotent cells and germ cells10. haploid embryonic stem (ES) cells in medaka fish4 and mice5,6 Most of the originally obtained ahES-cell lines contained approxi- raised the possibility of using engineered mammalian haploid cells mately 10–30% haploid cells. After three or more