巨噬细胞吞噬凋亡中性粒细胞的实时动态观察及其转归的研究.pdfVIP

Dynamic process of phagocytosis and fates of macrophages 1 after their ingestion of apoptotic neutrophils Wang Jiong, Huang Weilin, Wang Cheng, Liu Rongyu Department of Pulmonology, Anhui Geriatrics Institute, the First Affiliated Hospital of Anhui Medical University, Hefei, Anhui ( 230022) Abstract Clearance of apoptotic neutrophils by macrophages is important for the successful resolution of acute inflammation and homeostasis. In this study, the dynamic process of phagocytosis of apoptotic neutrophils was measured in real time and special attention was paid to the fates of macrophages after their phagocytosis in vitro, we staged the recognition and tethering, internalization, digestion and exocytosis stages of phagocytosis of apoptotic neutrophils. Furthermore we found that some macrophages underwent cell death after their ingestion of apoptotic cells. The dead macrophages consisted of autophagy, apoptosis and oncosis as revealed by transmission electron microscopy and confocal microscopy combined with specific dyes. These results demonstrated that after ingestion of apoptotic neutrophils, macrophages might undergo autophagy and apoptosis. Autophagy of macrophage after ingestion of apoptotic cells may be one of the new mechanisms in immune response. Keywords: macrophage; cell death; phagocytosis; time-lapse imaging 1. Introduction Respiratory infectious disease is a common and leading cause of morbidity and mortality worldwide [1]. The acute response to