应用RTPCR方法快速诊断猪繁殖与呼吸综合征的研究.pdf

吉林农业大学学报 2010, 32( 6) : 666~ 669 htt :/ / xuebao.j lau. edu. cn Journal of Jilin Agricultural University E-mail :j lndxb@ vi . sina. com RT- PCR 1, 2 1** 2 2 2 1 2 王 鑫 , 周铁忠 , 李 菲, 段晓波 , 王 开, 高慎阳, 胡桂学 1. 辽宁医学院畜牧兽医学院, 锦州121001; 2. 吉林农业大学动物科技学院, 长春130118 : 参照国内外已发表的猪繁殖与呼 综合征病毒(PRRSV) 的ORF5 基因序列设计1 对引物, 建立检测 PRRSV 的RT- PCR 方法, 并对长春周边不同地区10 个猪场送检的20 份病料进行检测特异性试验敏感性 试验和重复性试验结果表明, 此方法可以用于临床检测 PRRSV应用该方法从3 个猪场的4 份病料检出 PRRSV, 证实长春周边地区存在PRRSV 感染 : 猪繁殖与呼 综合征病毒; ORF5 基因; 反转录 聚合酶链式反应 : S85828; S8553 : A : 1000-5684( 2010) 06-0666-04 Rapid Diagnosis of Porcine Reproductive and Respiratory Syndrome with the RT- PCR 1,2 1 2 2 2 1 WANG Xin , ZHOUTie-zhong , LI Fei , DUANXiao-bo , WANG Kai , GAO Shen-yang , HU Gu-i ue2 1. College of Animal Science and Veterinary Medicine, Liaoning Medical University, Jinzhou 121001, China; 2. College of A nimal Science and Technology , Jilin Agricultural University, Changchun 130118, China Abstract : This research has esigne a pair of primers base on the ORF5 gene of the PRRSV sequences publishe to set up a RT- PCR metho in or er to etect PRRSV. We have etecte 20 pathologic mate- rials via this metho from 10 ifferent pig farms aroun Changchun. It has been in icate that the etect- ing metho of RT- PCR can apply to clinical etection through specificity test, sensitivity test an re- peatability test. The results of this research etecte 4 pathologic materials PRRSV positive from 3 farms confirming that PRRSV infection oes exist aroun Changchun. Key words: porcine repro uctive an respiratory syn rome vir