之融合蛋白多变异区基因分析(论文资料).pdfVIP

犬瘟熱病毒之融合蛋白多變異區基因分析 疫學研究組 李敏旭 副研究員 摘要 犬瘟熱是一種分布在全世界的高傳染性疾病，病毒的血球凝集素 及融合蛋白基因的變異與犬瘟熱發生的頻率相關，但相對於血球凝集 素基因，對於融合蛋白基因的變化則知道很少，本研究則透過收集台 灣犬瘟熱病例之融合蛋白全基因序列之分析來探討，在基因親緣性分 析上，這些病例大部分相近於鄰近台灣的中國大陸及日本病毒株，但 與疫苗株則有明顯的差異，值得注意的是主要變異區發生在前鉎肽 區，本土株間的序列相似度在89﹪，但與疫苗株的相似度在64-67﹪， 而花蓮所收集到之病毒株因地理上區隔自形成一個新的基因分支。 The identification of frequent variations in the fusion protein of canine distemper virus Ming-Shiuh Lee Abstract Canine distemper (CD) is a highly contagious disease with a worldwide distribution. Genetic diversity in genes encoding the haemagglutinin (H) and fusion (F) virus envelope proteins have been implicated in the increasing incidence of CD. Unlike the H gene, little is known about the genetic variability of the F gene in this virus. In the present study sequence analysis of the complete coding region of the F protein from CD virus isolates from Taiwan were carried out. Phylogenetic analysis demonstrated that the majority of isolates were similar to those found in neighbouring China and Japan, but were genetically distinct from vaccine strains. Remarkable variations were found scattered throughout the pre-peptide region (residues 1-135). The sequence identity of this region between locally sourced strains and between these strains and vaccine strains was 89% and 64 to 67%, respectively. Analysis suggested a novel strain of distant genetic lineage was present in dogs in the geographically isolated city of Hualien.