山东道地药材金银花三种品系鉴别研究.pdf

T h e Id cn tified R e sea r eh o f T h r ee C u ltiv a r s o f F lo s L o n icer a e jaP on icae of th e fam ou s一reglon C h in ese M ateria M ed ica in S h a n d o n g P ro v in ce S P eeia lity : C hinese M ateri a M ed iea A u th o r : M 厄n g 叭/en b in g Tu tor : P ro f. L i F en g A b str a ct O bjeetive : Th e aim of thi s Paper w as to Provide seientifie data for the quality stna dard of Shandon g Provincial F los L onicerae jaPonieae ，thr ee cu ltivars of its wre e studied on original，m aeroseoP ieal an d m icro scoP ieal ident ifiea一tion :on H P L C fingepr rint atl a s an d R A P D fin g erpr in t atla s. M e tho d s :Th e id en tified ch ara eteri stie o f the th ree cu ltiv ars w ere o b ta in ed b y C h in ese M ateri a M ediea origni al，m acro seoP ieal and m icro seoP ieal identifi一cation ，H P L C and R A P D atla s. R esu lts:O riginal，m acroseoP iealand m icro seoP iealidentifieation of tl甘ee eu ltivars of Shandong Provineial F los L onicerae jaPonieae w ere identified.H P L C fingerpn ni show ed :there w as 82.4% sim ilari ty b etw een Jizhuahua cu ltivar and S ijihu a cu ltivar ; 77.4% sim il丽 ty betw een M aohu a cu ltivar and Sijihu a cu ltivar; 55.5% sim il如 ty b etw een M ao hu a cu ltiv ar an d J izh u ah u a cul tiv ar . Th e co n ten ts o f C h lo ro g en ic a c id an d luteolin一7一p一D 一glucoside w ere analysed by H PL C ，the resu lts are (M aohu a cul tivar) (Sijihu a cultivar) (Jizhu ahua cu ltivar) .B iologiealfingepr rintshow ed :Prim er SB SD 06 、 S B S B 19 g en erated d istin ct fin g epr rin tin g P att em s that eou ld d istin gu ish each o f t