水稻H3.2型组蛋白基因RH3.2A的克隆与盐胁迫下的表达分析.pdfVIP

遗 传 学 报 Acta Genetica Sinica , September 2006, 33 (9) ：833–840 ISSN 0379-4172 Salt Induces Expression of RH3.2A, Encoding an H3.2-type Histone H3 Protein in Rice (Oryza sativa L.) QIU Sheng-Ping, HUANG Ji, PAN Li-Juan, WANG Mei-Mei, ZHANG Hong-Sheng① State Key Lab of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China Abstract: Histone H3 is one of the four histones, along with H2A, H2B, and H4, which form the eukaryotic nucleosome octamer core. In this study, a new gene RH3.2A encoding an H3.2-type histone H3 protein from rice (Oryza sativa L.) was reported. RH3.2A was cloned through RT-PCR from salt-treated rice seedlings. This gene encoded a protein of 136 amino acid residues that were similar to some plant histone H3 proteins reported previously. However, the cDNA sequence of RH3.2A and other rice H3 genes were different. Alignment of RH3.2A encoding protein with other plant histone H3 proteins revealed that three amino acid residues (32, 88, and 91) were markedly different between H3.1-type and H3.2-type proteins. The mRNA expression analysis of RH3.2A revealed that RH3.2A gene was upregulated by salt stress in rice roots and ABA treatment in seedlings. The potential role of RH3.2A during salt stress was discussed. Key words: rice; gene expression; histone H3; salt stress Histones are one of the major structural compo- named as replacement histones. The main difference nents of cells, participating in the packaging of DNA [1]. between the H3.2 and H3.1 genes is the presence of Several types of histones including H2A, H2B, H3 introns in the H3.2 variants [1]. Besides, the H3.1 genes and H4 interact to form the eukaryotic nucleosome in animals lack mRNA polya