《MicroRNA and histopathological characterization of pure mucinous breast carcinoma》.pdfVIP

Cancer Biol Med 2013;10:22-27. doi: 10.7497/j.issn.2095-3941.2013.01.004 ORIGInaL aRTICLE MicroRNA and histopathological characterization of pure mucinous breast carcinoma 1* 2* 1 1 1 Feng Zhou , Shuai Li , Hui-Min Meng , Li-Qiang Qi , Lin Gu 1 2 Departments of Breast Oncology/Surgery; Department of Breast Cancer Pathology and Research Laboratory, Tianjin Medical Univer- sity Cancer Institute and Hospital; Key Laboratory of Breast Cancer Prevention and Therapy, Ministry of Education; Key Laboratory of Cancer Prevention and Therapy, Tianjin; State Key Laboratory of Breast Cancer Research, Tianjin 300060, China AbstrAct Objective: Pure mucinous breast carcinoma (PMBC) is an uncommon histological type of breast cancer characterized by a large amount of mucin production. MicroRNA (miRNA) is a large class of small noncoding RNA of about 22 nt involved in the regulation of various biological processes. This study aims to identify the miRNA expression profile in PMBC. Methods: MiRNA expression profiles in 11 PMBCs were analyzed by miRNA-microarray and real-time polymerase chain reaction (PCR). Thirty-one PMBCs and 27 invasive ductal carcinoma of no special types (IDC-NSTs) were assessed by immunohistochemistry using antibodies against ER, PR-progesterone receptor, HER2, Ki-67, Bcl-2, p53, PCNA, and CK5 and 6. Results: We analyzed the miRNA expression in 11 PMBCs and corresponding normal tissues using miRNA-microarray and real-time PCR, and found that miR-143 and miR-224-5p were significantly downregulated in mucinous carcinoma tissue. Compared