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septc渤:Ⅲv“:。…94,
Availableonlineat
JournalofNortheast
AgriculturalUniversity(EnglishEdition)
LentivirusMediatedGene in ofPorcine
Trophectoderm
Manipulation
Embryos
Liu
Yin Shi.chao,Mu Zhong-hua*
Zhi,GuoJia,BouGerelchimeg,LiuYan—shuang,and
ofLifeSciences,NortheastUniversity,Harbin150030,China
College Agricultural
oftoolsthatcan and inthe
expressionspecificallyefficiently
Abstract:Developmentmanipulategene
first
aid therolesofdifferent inTEversus showed
would embryoniclineages.Here,we
greatlyunderstanding geneticpathways
to of inTEcells.
that lentivirusinfectionof couldlead
time short-term porcineblastocysts rapidexpressiontransgenespecifically
III-drivenshort
knockdowncouldalsobeachievedlentivirus-mediated
Efficient pol hairpin
TE.specificgene by
with lentivirus
couldbe controlled this Tet-On
efficientlycombiningsystem system.This
TE.specificgeneexpressiontemporal by
wouldfacilitatefunctionstudiesin knockdown
infection
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