《Levels of biological markers of nitric oxide in serum of patients with squamous cell carcinoma of the oral cavity》.pdfVIP

International Journal of Oral Science (2013) 5, 141–145 2013 WCSS. All rights reserved 1674-2818/13 /ijos ORIGINAL ARTICLE Levels of biological markers of nitric oxide in serum of patients with squamous cell carcinoma of the oral cavity 1 1 2 3 Wioletta Ratajczak-Wrona , Ewa Jablonska , Bozena Antonowicz , Dorota Dziemianczyk and Stanislawa Zyta Grabowska3 The aim of the study was a determination of the levels of nitric oxide (NO) and its biological markers such as malonyldialdehyde (MDA) and nitrotyrosine in the serum of patients with squamous cell carcinoma (SCC) of the oral cavity and identification of the relationships between NO and those markers. These studies were performed on patients with SCC of the oral cavity before and after treatment. Griess reaction was used for the estimation of the total concentration of NO in serum. The nitrotyrosine level in serum was assessed with an enzyme-linked immunosorbent assay (ELISA) kit, and MDA level using a spectrophotometric assay. Higher concentrations of NO in blood serum were determined in patients with stage IV of the disease before treatment in comparison to the control group and patients with stages II and III of the disease. Moreover, higher concentrations of MDA and nitrotyrosine were determined in the serum of patients in all stages of the disease in comparison to healthy people. After treatment, lower concentrations of NO in the serum of patients with stage IV of the disease were observed in comparison to the amounts obtained prior to treatment. In addition, lower levels of n