Analysis of natural carbohydrate biopolymer-high molecular chitosan.pdfVIP

Carbohydrate Polymers 68 2007 511–516 /locate/carbpol Analysis of natural carbohydrate biopolymer-high molecular chitosan and carboxymethyl chitosan by capillary zone electrophoresis Xiaofang Fu a, Ling Huang b, Maolin Zhai b, Wei Li a, Huwei Liu a,¤ a Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China b Beijing National Laboratory for Molecular Sciences, Institute of Applied Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China Received 6 September 2006; received in revised form 28 October 2006; accepted 6 November 2006 Available online 11 December 2006 Abstract Chitosan and its derivatives have been widely used in many Welds for their various function, there were some diVerence in bioactivities and applications between them. In this paper, a direct capillary zone electrophoresis CZE method without any pre-treatment was devel- oped for separation of chitosan and its derivative, a water soluble chitosan called carboxymethyl chitosan CM-chitosan , which was proved to be rapid and eVective with satisfactory resolution. Optimization of conditions including pH, concentration of the buVer, and length of column were investigated, showing that when employing 50 mM sodium phosphate at pH 2.0 and untreated fused silica capil- lary, high-molecular weight chitosan and CM-chitosan were baseline separated with ultraviolet UV detector with satisfactory repeat- ability and excellent linear responses. Therefore, this method could be applied to separate and identify chitosan and CM-chitosan in biological samples and commercial products. ? 2006 Elsevier Ltd. All rights reserved. Keywords: Chitosan; Caboxymethyl chitosan; Capillary zone electrophoresis; Separation; Analysis 1. Introduction speciality of b