动物源性食品中六种氟喹诺酮类残留检测前处理的优化.pdfVIP

# 28# 20 11, 45( 4) : 28~ 32 / , 潘 媛, 牛 华, 程晓云, 祝红昆, 李 波, 冯 雷, 珠 娜, 牛之瑞 ( , 650223 ) [ ] 20 10- 08- 14 [] A [ ] 1002- 1280 ( 20 11) 04- 0028 - 05 [ ] S859. 84 [ ] 建立了一个检测动物源性食品 6 种氟喹诺酮类药物残留的高效液相色谱方法对不 同基质的样品前处理条件经行了优化: 鱼肉及肝脏样品经过乙腈- 0. 1 m ol/L 磷酸二氢钾缓冲液 提取, 正己烷洗涤去除油脂; 蛋及乳制品样品经过乙腈提取, 正己烷洗涤去除油脂目标化合物采 用高效液相色谱- 荧光检测器检测, 外标法定量添加 102050100 Lg / kg浓度水平时, 回收率在 82% ~ 105% 之间, 相对标准偏差在 4% ~ 12% 之间, 方法的检出限为诺氟沙星环丙沙星沙拉沙星 及单诺沙星为 5. 0 Lg / kg恩诺沙星达氟沙星为 3. 0 Lg / kg方法操作简单实用, 基质干扰小, 灵 敏度高, 可以满足动源性食品 氟喹诺酮类药物残留检测的需要 [] 高效液相色谱法; 氟喹诺酮; 残留; 检测; 动物源性食品 Pretreatm ent Optim ization on Determ ination of Six Fluoroquinolones in Animal Foods PAN Y uan, N IU H ua, CHENG X ao- yun, ZHU H ong- kun, L I Bo, FENG L e, ZHU N a, N IU Zh - ru (YunnanProvincial Institute of Product Quality Sup ervision and Insp ection, Kunming 650223; China) Abstract: A h gh performance l qu d chromatog raphy m ethod w as proposed for analyz ng 6 fluoroqu nolones( FQ s) n products of anm al t ssues. The sample pretreatm ent of d fferent substratesw ere opt m zed, f sh, m eat and l ver sam ples w ere extracted by CH CN - 0. 1mo l/L KH PO buffer solut on, eggs and da ry products ex tracted by CH CN, then 3 2 4 3 hexane w as added nto rem ove grease; target com pounds w ere detected by H PLC - flurorescence detector, and quant tat ve w th ex ternal standard m ethod. The recover es of 6 FQ s w ere 82% ~ 105% at four sp ked leve:l 10, 20, 50, 100 Lg / kg, and the RS were blew 12. 0% . The LOD o f norfloxac n, c pro floxac n, sarafloxac n and d floxac n w ere 5. 0 Lg / kg,