离子液体萃取甘草.pdfVIP

J. Sep. Sci. 2009, 32 , 4033–4039 4033 Minglei Tian Research Article Wentao Bi Kyung Ho Row Solid-phase extraction of liquiritin and Department of Chemical glycyrrhizic acid from licorice using ionic Engineering, Inha University, Incheon, Korea liquid-based silica sorbent Received July 20, 2009 A new ionic liquid-based silica sorbent was developed by a process involving surface Revised August 20, 2009 chemical modi?cation of commercial silica using a synthesized ionic liquid. The obtained Accepted September 8, 2009 particles were successfully used as a special sorbent in a solid-phase extraction process to isolate liquiritin and glycyrrhizic acid from licorice. Different washing and elution solvents, such as water, methanol/water v/v , and pure methanol were evaluated. Ionic liquid-based silica sorbent was compared with traditional C18 sorbent and it exhibited higher selectivity. Quantitative analysis was carried out by using a C18 column. Good linearities of two compounds were obtained from 5 104 to 0.2 mg/mL r2 40.99 with the relative standard deviations o1.0%. The target compounds in commercial herbal medicines containing licorice were determined, and the bound rates between the target compounds and protein were obtained by this sorbent. Keywords: Glycyrrhizic acid / Ionic liquid-based silica / Licorice / Liquiritin / Solid- phase extraction DOI 10.1002/jssc.座机电话号码7 1 Introduction procedure and increase the amounts extracted. Otherwise, as a medicine for human, pharmacological test, such as Licorice is the root of the glycyrrhiza plant species that has bound rate with protein should be established. Also the been used medicinally for more than 4000 years [1]. It is a testing method needs to be developed [17, 18]. Chinese herb that is commonly used as an expectorant and Solid-phase extraction SPE has been developed as an to arrest coughing,