响应面分析法优化枸杞多糖提取工艺条件.docVIP

响应面分析法优化枸杞多糖提取工艺条件【摘要】? 　　目的利用响应面分析法（Response Surface Methodology）对枸杞多糖的提取工艺进行优化。方法在单因素实验基础上选取实验因素与水平，根据中心组合（Box-Benhnken）实验设计原理采用3因素水平的响应面分析法，依据回归分析确定各工艺条件的影响因子，以多糖提取率为响应值作响应面和等高线。结果在分析各个因素的显著性和交互作用后，得出枸杞多糖水浸提的最佳工艺条件为：料液比132；浸提温度84；浸提时间2.3h；浸提1次。结论在最佳工艺条件下，枸杞多糖的实际提取率可达11.10%。 【关键词】? 枸杞 枸杞多糖 提取 响应面分析 　　Optimization of the Extraction Technology of Lycium barbarum Polysaccharide by Response Surface Methodology 　　LIU Junhai， REN Huilan，LI Fengfeng，LI Guanglu 　　1.College of Chemistry and Chemical Engineering，Liaocheng University，Liaocheng 252059， China; 　　2.Department of Chemistry of College of Science, Zhejiang University,310027,Zhejiang, China 　　Abstract：Objective To optimize the extraction technology of Lycium barbarum polysaccharides by response surface methodoloy.MethodsExperiment factors and levels were firstly selected by one factor tests. On the basis of the previous results and according to the central composite experimental design principles,the method of Response Surface Method with 3 factors and 3 levels was adopted.Then the factors influencing the technological parameters were determined by means of regression analysis.Response surface and contour were finally graphed with the extraction rate as the response value.ResultsThe optimum conditions of Lycium barbarum polysaccharides extraction were as follows:the ratio of material to solvent of 1∶32,extraction temperature of 84℃ and extraction time of 2.3 h,one time extraction.ConclusionThe yield of Lycium barbarum polysaccharides is 11.10% under the optimum conditions. 　　Key words：Lycium barbarum ;? Lycium barbarum polysaccharides ;?? Extraction;? Response surface methodology 　　枸杞是多年生灌木，花紫、果红、枝繁叶茂，具有滋补肝肾、益精明目的功效［1］。枸杞子是我国传统中药，具有很高的药食两用价值。近年来，枸杞作为一种新兴的保健型食用植物在我国宁夏、新疆等地区进行了大面积种植，种植面积逐年增加。众多的研究表明，枸杞多糖具有保肝、降血糖、降血脂、抗肿瘤、抗衰老等多种生理功效［2～7］。因此，提取枸杞多糖对开发和利用枸杞资源具有重要意义。 　　响应面分析(Response Surface Methodology)法系采用多元二次回归方法作为函数估计的工具，将多因子实验中因素与指标的相互关系用多项式近似拟合，依此可对函数的响应面和等高线进行分析，研究因子与响应面之间、因子与因子之间的相互关系。它与过去广为使用的“正交实验设计法”不同，具有实