通气量与稀释率对nisinZ发酵的影响.pdfVIP

International Dairy Journal 11 (2001) 943–951 Effect of aeration and dilution rate on nisin Z production durin continuous fermentation with free and immobilized Lactococcus lactis UL719 in supplemented whey permeate P. Desjardins, J. Meghrous, C. Lacroix* ! ! Dairy Research Centre STELA, Pavillon Paul-Comtois, Universite Laval, Quebec, Que., Canada G1K 7P4 Received 5 February 2001; accepted 4 August 2001 Abstract The influence of dilution rate (D) and aeration on soluble and cell-bound nisin Z production was investigated durin gcontinuous free (FC) and immobilized cell (IC) cultures with Lactococcus lactis subsp. lactis biovar diacetylactis UL719 in supplemented whey permeate. Maximum total bacteriocin titres durin gnon-aerated continuous FC and IC cultures were obtained for low D; with 1490 and 1090 IU mL1 for 0.15 h1 or 0.25 and 0.5 h1, respectively. For both systems, aeration increased nisin total production with maximum titres of 2560 and 2430 IU mL1 for low D; respectively, as well as specific production. Volumetric productivity was the highest for an intermediate D of 0.4 h1 durin gFC cultures (460 IU mL1 h1 for both aerated and non-aerated cultures), while it increased continuously with D durin gIC cultures, reachin ghigh values of 1090 and 1760 IU mL1 h1 at 2.0 h1 without and with aeration, respectively. In comparison with previous data for FC batch cultures, data from this study may indicate that durin continuous fermentations at steady state, some steps in nisin biosynthesis are limiting. In these conditions, nisin production by immobilized cells is reduced. r 2002 Elsevier Science Ltd