猪源肠外致病性大肠杆菌Ⅵ型分泌系统基因vca0107基因缺失突变株的构建及其生物学特性的研究.pdf

猪源肠外致病性大肠杆菌Ⅵ型分泌系统基因vca0107基因缺失突变株的构建及其生物学特性的研究.pdf

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83.1.553.2.53.2.63.2.971.31.3.11.3.21.4.21.4.31 4.2.14.64.7 Escherichia07 whichwidewhichwidewaterwithVWeABSTRACTmedicalmoremadementionedVASH7DbebebebacteriabackbacteriabeenPCN033butPCN06PCN033abasisrecentnotnotExtraintestinalEscherichiahostExPEChaveExPECEcoliExPECExPEChosthostT6SST6SST6SShasT6SST6SSPCN033.WeaaaaharmharmhealthresearchnewroalononeoverhumanvirulenceviavirulentvirulentOvca一0107newborns.researchreaserchrecombinationatCanCanCancanandattentionareareattenuatedafterevenofofofofofofofofoutbreaksofofofofofofofofofofofofofofofofofanimals.Toasashumancontents2—25othertracttoettofiscalfoundfoodfactorfarfunctionfunctionfromfuctionandandandandandandandandandandandandandanandand25%ofcolidifferentcellcellcontainconserved.Theclear.discoverchoosedeletedfromtissuesthetissueseffortthanthethetranslatedthethethedeathclusterthethethethethethethethethethethethethetoxinsthethenthethethethatthethethethethethethetheclusterfoundfoundstudiesshouldstockswinesecretionsixsecretionsecretionstrainstrainstrainswinesuicidecharacteristics.Thesomelivestockllinfectionintestinalitsintestinalinfectionsisisinisinintointeractitsisinitsisisinininisitsleast111.Constructioninduse werewerewaswhichwerewhichwholewitllwerewerewewaswaswerewithwimwithmutantsmutantsmutantmicemutantmutantnoPCN033PCN033PCRPCN33Avca0107PRElPCN033.AfterPCR.ThisbeenPCRPCRbacterialPCN03FemaleusedPCN033PCN03PK-15PCN03PCN03anotTheE.coilX72hadh.NohasEscherichiaKMvitroarlnhasLBCm6resultbetweenonvcaOl07vcaOl07verificationvca一01vca-0107.OD600vca-0107recombinationPCN033.TheatrecombinantRAWcrossovercurvesandandandandandandadhesiveareofofofofof0707ofofofof07.ofofofobviousobservedofofofPCN033.of0707correct22Avca-0toeventtotototoflanksforfirstfirstforandandandcloneddoubleconnectedclonedcontainedcontained50donor3clonesdidn’tcontainedcoloniesdeletedcharacteristicsculturesdetermineddifference3deletioncellcelldifferences3Avca-03.fromthetwothethethethethethethethethethethethethethethatthethethethethethethechromosome.Coloniesdeterminethet

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