《北京暑期学校-彭练矛-electron-microscopy》.pdfVIP

Introduction to Electron Microscopy Introduction to Electron Microscopy Prof. David Muller, dm24@ Rm 274 Clark Hall, 255-4065 Ernst Ruska and Max Knoll built the first electron microscope in 1931 T4 Bacteriophage (Nobel Prize to Ruska in 1986) Electron Microscopy bridges the 1 nm – 1 μm gap David Muller 2008 between x-ray diffraction and optical microscopy Tools of the Trade Tools of the Trade AFM MFM Scanned Probe Microscope (includes Atomic Force Microscope) Transmission Electron Microscope Scanning Electron Microscope David Muller 2008 Biological and Electronic Component Dimensions Biological Electronic Components Tool 1 Logic Board 10-2 Computer chip SEM Optical 10-4 Mammalian cell Microscope ) m ( e z i S Bacterial cell 10-6 TEM Transistor Virus AFM/STM 10-8 Gene Gate Oxide Protein 10-10 Atom David Muller 2008 Comparison of Optical and Electron Microscopes Comparison of Optical and Electron Microscopes • Electron microscopes are operated in vacuum because the mean free path of electrons is air is short – this mean biological samples should not degas – they can either be dehydrated or