《经瞳孔温热疗法对大鼠视神经钳夹视网膜神经节细胞的保护作用2016年论文》.pdfVIP

眼科研究2009年 5月第 27卷第 5期 385 - · 实 验 研 究 · 经瞳孑L温热疗法对大 鼠视神经钳夹视网膜神经节 细胞 的保护作用 刘学群 姜利斌 李志华 张 岐 马 瑾 谢 君 刘 磊 TheprOtectiVe effelcts0ftranspupillary therm 0therapy On retinalgangli0n cellsin ratwith 0pticnerVecrush ，J eqn，l， ，g 6n，L Z M口，Z 0凡g 凡g， 0I，n， et，n，￡M e．曰e ，g bng九?nEyeCe凡fer，Be ng 0|ngren P ￡0Z，Cc 。Z cⅡZ e， y，Be ，g DD73D，C 口 Abstract objectiVe Thisstudywasdesignedtoexplorethepr0tectiveefflect0fsubthresholdtranspupillaythemotherpy (TTT)0nretinalgang1ioncells(RGCs)intheratwithpanial0pticnervecrush． Methods 810nm diodelaserwasusedto irradiatetheretinasurroundingopticnerve(5spots)f0r3Osecondsintheleft。yes0fBNrats．Threedayslater，thepanialoptic nervecrush modelswerecreated in the ratsby clamping the retrobulbaroptic nerve f0r60 sec0nds．102 male BN ratswere randomlydividedintoTTTwith0pticnervecrush(TTT+0NC)gr0up，0pticnervecrush(0NC)group，TTTwithsham 。peration (TTT)groupandnormalc0ntro1group．RGCsweredetectedbytheretrogradelabel0fnuorog0ldatl，2，4weeksafter。pticnerve crush．HSP70 expressi0nwasassessedusing immun0hist0chemistryandW esternblotin 12ratsunderwentTTT irradiation．The hist0l。gicalexaminationofratsretinawith TTT irradiati0nwa peIformedunderthelightandtransmission electronmicroscope． Results TheRGCsdensiyin0NCgr0upwas(863±87)cells／mm at1weekanddecreasedt0(6l4±l24)at2weeksand (424±112)ceUs／mm at4weeksafteriⅡadiati0n．InTTT+0NCgroup，thedensityofRGCswas(8l9±93)ceIls／mm atlweek anddecreasedto(623±123)at2weeksand(6l5±112)cel1s／mm at4weeks．TheRGCsdensiyat4weeksin0NCgroup wassignificantlyl0werthaninTTT+ONCgroup(P=0．0O6)．Theexpressi0nofHSP7OintheretinaunderwentTTTirradiation washigherthanthef110w ye．Thethinning ofretinaand degranulati0n ofretinalpigmentepithelialcellsweref0und underth lightmicrosc0pe，andshri