应用斑点金免疫渗滤试验快速同步检测抗HIV1HIV2 IgG抗体.pdfVIP

21 2 21 2116-120 2006 3 VIROLOGICA SINICA March 2006 HIV-1HIV-2 IgG ** * ( 430072) Rapid and Simultaneous Detection of IgG Antibodies to HIV-1 and HIV-2 by Dot Immunogold Filtration Assay ** ZHAI Jian-xin, WANG Ye-fu , DUAN Lian-lian, XU Zheng-hui, WAN Zhi-xiang, YANG Yong (College of Life Sciences, Wuhan University, Wuhan 430072, China) Abstract A simple rapid detection of HIV-1 and HIV-2 antibodies was developed by using dot immunogold filtration assay. Five recombinant proteins derived from HIV env and gag regions of HIV-1 and HIV-2 (P24, GP41, GP120C, GP120V3 and GP36) were expressed in E. coli . The proteins were purified, immobilized onto the nitrocellulose membrane and used for detecting anti-HIV IgG antibodies Human IgG was used as internal control. Anti-HIV antibodies detected in sera by these antigens were observed with the naked eye with the aid of colloidal gold labeled SPA. A total of 51 sera were tested by ELISA and by our rapid HIV assay. A total of 21 HIV positive samples (containing one HIV-1 positive control serum and one HIV-2 positive control serum) confirmed by Western blots exhibited a positive reaction when tested by our assay. HIV negative sear (30 samples) were also negative by our test. These results suggested that our method can be used for detecting anti-HIV antibodies and has the advanteage of quickness, simplicity and cost effectiveness. In addition, simultaneous detection and discrimination among four HIV markers (anti-P24, GP41, GP120 and GP36 antibodies) by the rapid HIV test would be useful for increasing the sensitivity and accuracy of this rapid test and fo