《SOD对表没食子儿茶素没食子酸酯抗肿瘤活性及稳定性的影响》.pdfVIP

蓑叶科学2007，27(4)：293—298 TeaScience Journalof SOD对表没食子儿茶素没食子酸酯 抗肿瘤活性及稳定性的影响 张斌，夏艳秋，金礼吉，徐永平 (大连理工大学环境与生龠学院生翰科学与工程系，辽宁大连1t6024) 和人官颈癌细胞Hela生长的影响。通过检测培养基中乳酸脱氢酶的活力研究EGCG对三种细胞的细胞毒作用． 以上研究均设加入SOD和不加SOD两个对照组。研究表明；与投有SOD存在条件下相比，加入SOD后，细胞 存活率下降，培养基中LDH活性增强．HT-29细胞凋亡宰上升；EGCG在培养基中的稳定性增强。 关t词：EGCG；SOD；HT-29细胞 中囤分类号：$571．1；R983 文献标识码：A EffectofSODonAnti—cancerand Activity of Stability ZHANG Bin。XIAYan-qiu。JINLi-ji，XU Yong—ping and ofBioJcieaceand ofofEavitonmemtl (Deptnmem Biotechaology．schooI B“晒cal$cieace of ofhumancolonic cells Abstract：Effects the CancerHT-29，human epigalIocatechin·3一gallate(EGCG)ongrowth breastclmcerMCF-7andhumancervicalcancerHelaWere MTT ofEGCGOil investigatedby assay．Cytotoxicity thesecoilsWaS cell detectedbydeterminingtheactivityofLDHin atftertheEGCGtreatmentwas flow of inDMEM／F-12Wit8determined analyzed EGCG by cytometry．Stability by HPLCandallgudiesabovewere inthe orabSanCCof showedthatceils comparedpresence SOD．Investigation survival culture after deceasedand ofLDHinthe meditimincreased SOD．m percentage activity adding apoptofic r出ofHT-29andthe ab$cncoofSOD． stabilityofEGCGincult