2002-禽、猪、人戊型肝炎-美国-孟祥金.full.pdfVIP

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2002-禽、猪、人戊型肝炎-美国-孟祥金.full.pdf

Journal of General Virology (2002), 83, 2201–2209. Printed in Great Britain The putative capsid protein of the newly identified avian hepatitis E virus shares antigenic epitopes with that of swine and human hepatitis E viruses and chicken big liver and spleen disease virus G. Haqshenas,1 F. F. Huang,1 M. Fenaux,1 D. K. Guenette,1 F. W. Pierson,1 C. T. Larsen,1 2 1 1 H. L. Shivaprasad, T. E. Toth and X. J. Meng 1 Center for Molecular Medicine and Infectious Disease, Department of Biomedical Sciences and Pathobiology, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, 1410 Price’s Fork Rd, Blacksburg, VA 24061-0342, USA 2 California Animal Health and Food Safety Laboratory System, School of Veterinary Medicine, University of California-Davis, 2789 South Orange Ave, Fresno, CA 93725, USA We recently identified a novel virus, designated avian hepatitis E virus (avian HEV), from chickens with hepatitis–splenomegaly (HS) syndrome in the USA. We showed that avian HEV is genetically related to swine and human HEVs. Here we report the antigenic cross-reactivity of the putative open reading frame 2 (ORF2) capsid protein of avian HEV with those of swine and human HEVs and the Australian chicken big liver and spleen disease virus (BLSV). The region encoding the C- terminal 268 amino acid residues of avian HEV ORF2 was cloned into expression vector pRSET-C. The truncated ORF2 protein was expressed in E. coli as a fusion protein and purified by affinity chromatography. Western blot analysis revealed that the avian HEV ORF2 protein reacted with antisera against the Sar-55 strain of human HEV and with convalescent antisera against swine HEV and the US2 strain of human HEV, as well as with antiserum against BLSV. Convale

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