- 23
- 0
- 约3.44万字
- 约 9页
- 2015-11-10 发布于安徽
- 举报
Journal of General Virology (2002), 83, 2201–2209. Printed in Great Britain
The putative capsid protein of the newly identified avian
hepatitis E virus shares antigenic epitopes with that of swine
and human hepatitis E viruses and chicken big liver and spleen
disease virus
G. Haqshenas,1 F. F. Huang,1 M. Fenaux,1 D. K. Guenette,1 F. W. Pierson,1 C. T. Larsen,1
2 1 1
H. L. Shivaprasad, T. E. Toth and X. J. Meng
1 Center for Molecular Medicine and Infectious Disease, Department of Biomedical Sciences and Pathobiology, College of Veterinary
Medicine, Virginia Polytechnic Institute and State University, 1410 Price’s Fork Rd, Blacksburg, VA 24061-0342, USA
2 California Animal Health and Food Safety Laboratory System, School of Veterinary Medicine, University of California-Davis,
2789 South Orange Ave, Fresno, CA 93725, USA
We recently identified a novel virus, designated avian hepatitis E virus (avian HEV), from chickens
with hepatitis–splenomegaly (HS) syndrome in the USA. We showed that avian HEV is genetically
related to swine and human HEVs. Here we report the antigenic cross-reactivity of the putative
open reading frame 2 (ORF2) capsid protein of avian HEV with those of swine and human HEVs and
the Australian chicken big liver and spleen disease virus (BLSV). The region encoding the C-
terminal 268 amino acid residues of avian HEV ORF2 was cloned into expression vector pRSET-C.
The truncated ORF2 protein was expressed in E. coli as a fusion protein and purified by affinity
chromatography. Western blot analysis revealed that the avian HEV ORF2 protein reacted with
antisera against the Sar-55 strain of human HEV and with convalescent antisera against swine HEV
and the US2 strain of human HEV, as well as with antiserum against BLSV. Convale
您可能关注的文档
最近下载
- 河北广谋新材料科技有限公司年产600万平方米复合革及隔音布、400万平方米新型复合网项目环境影响报告表.docx VIP
- 部编版语文五年级下册第七单元教材解读大单元集体备课.pptx VIP
- 部编版语文五年级下册第六单元教材解读大单元集体备课.pptx VIP
- 部编版语文五年级下册第五单元教材解读大单元集体备课.pptx VIP
- 部编版语文五年级下册第四单元教材解读大单元集体备课.pptx VIP
- 部编版语文五年级下册第三单元教材解读大单元集体备课.pptx VIP
- 部编版语文五年级下册第二单元教材解读大单元集体备课.pptx VIP
- 部编版语文五年级下册第一单元教材解读大单元集体备课.pptx VIP
- 车工 (数控车床)理论知识考核要素细目表四级.pdf VIP
- 本科课程《基础护理学》教案,第十二章给药.doc VIP
原创力文档

文档评论(0)