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《Anatomical Study on the Multi-Ovule Development and Abortion of Hanfu Apple》.pdf
JournalofInte rativ
g— eAn。h。 蛳 ScienceDiirect A
2,no1144 1133r4、:·77770f1—777777 n。ri121n0l14
, (4)
AnatomicalStudyontheMulti—Ovule DevelopmentandAbortionof
HanfuApple
YANGXu—yuan,MAHuai—yu,LIUGuo—cheng,LODe—guo,QINSi-junandDUGuo.dong
CollegeofHorticultureShenyangAgriculturalUniversity/KeyLaboratoryforNorthernFruitTreesCultivationandPhysio—Ecologyof
ShenyangCity,Shenyang110866,PR.China
Abstract
Theterminalflowerbudsof6-yr—oldHanfuapplewereusedtostudytheovuledevelopment,ovularcharacteristics,cell
deathofabortiveovules,anddynamicchangeofstarchgrainquantityintheembryosacwithparaffinslicesandtemr inal
deoxynuc1eotidy1transferase—mediatedfluoresceindeoxyuridinetriphosphatenick—endlabeling(TUNEL)system.Four
anatropousovulesineachventriclecouldbeobservedbeforeflowering.W iththedevelopingoffloralorgan,thebulkof
nomr alovulesenlarged ineachventricle,thematureembryosacdifferentiated intonucellus,andtheeggcelldeveloped
intozygotebydoublefertilization.A largenumberofstarchgrainswereobservedduringpollentubergowthanddouble
fertilization,whichguraanteedbasicnurtientsupplyinthenomr aldevelopmentofovules.Moreover,abortionphenomenon
ofruntishouv lesemergedatthestagesofmautreembryosac,doublefertilizationandzygotedevelopment.Theabortion
characteristicsincludeddefomr iyt ofovuledevelopment,degradationofnucellustissue,separationbetweenfuniculusand
ouv le,abnomr alityoffour-nucleateembryosac,aswellasdevelopmentinterruptionofmatureembryosac.TUNELanalysis
provedthatouv leabortionwasprogrammedcel1death.
Keywords:Hanfuapple,multi—ouv le,abortion,programmedcelldeath,starchgrain
pollination. Thelargestnumberofseedperfruitof
INTRODUCTlON self-
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