《Application of Cydia pomonella expressed sequence tags: Identification and expression of three general odorant binding proteins in codling moth》.pdfVIP
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《Application of Cydia pomonella expressed sequence tags: Identification and expression of three general odorant binding proteins in codling moth》.pdf
InsectScience(2013)20,559l-574,DOI10.1111/j_1744—7917.2012.01560x
ApplicationofCydiapomonellaexpressedsequencetags:
IdentifiCatiOnandexpressionofthreegeneralodorant
blndInqprotelnslncodlingmoth
一 _
Stephen F.Garczynski’ BradS.Coates2 ThomasR.Unruh’ ScottSchaeffer3,DerickJiwan3,
, , ,
TysonKoepke3andAmitDhingra3
’USDA-ARS,YakimaAgriculturalResearchLaboratory,Wapato,WA98951 USDA-ARS,CornInsectandCropGeneticsResearchUnit,
GeneUcsLabora~ry,IowaStateUnivers.ty.Ames.IA50011and3DepartmentofHorticultureandLandscapeArchitecture.Washington
StateUniversity,PuHman WA 99164,USA
Abstract Thecodlingmoth,@ diapomonella,isoneofthemostimportantpestsof
pomefruitsintheworld,yetthemoleculargeneticsandthephysiologyofthisinsectremain
poorlyunderstood.A combinedassemblyof834lexpressedsequencetagswasgenerated
from Roche454GS.FLX sequencingofeighttissue.specificcDNA libraries.Putative
chemosensoryproteins(12)andodorantbindingproteins(0BPs)(18)wereannotated,
whichincludedthreeputativegeneral0BPfG0BP),onemorethantypicallyreported
forotherLepidoptera.To furthercharacterizeCpomGOBPs.wecloned cDNA copies
oftheirtranscriptsanddeterminedtheirexpressionpatternsinvarioustissues.Cloning
andsequencingofthe698]atrtanscriptforCpomGOBP1resultedinthepredictionofa
163aminoacidcodingregion.andsubsequentRT_PCR indicatedthatthetranscriptswere
mainlyexpressedinantennaeandmouthparts.The1289ntf160aminoacid1CpomGOBP2
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