《VEGFA,HIF1A和EPAS1基因多态性与猪繁殖性状的相关研究》.pdfVIP

第 32卷第 3期 上 海 交 通 大 学 学报 (农 业 科 学版) Vo1．32No．3 2014年 6月 JOURNALOFSHANGHAIJIAOTONGUNIVERSITY (AGRICULTURALSCIENCE) Jun．2014 文章编号：1671—9964(2014)03—0007—08 D0I：10 ． 3969／J．ISSN．1671—9964．2014．03．002 PolymorphismsoftheSwine VEGFA ，HIF1A andEPAS1 GenesandTheirCorrelationwithReproductiveTraits ZHANGXiang—zhe 。，YANGLun。，CHEN Qiang ，TANGui一 ng ， CHEN Jian ，YAN Guo—xiang ，PAN Yu—chun1,3 (1．SchoolofAgricultureandBiology，ShanghaiJiaotongUniversity，Shanghai200240，China； 2．Bio-XCenter，ShanghaiJiaotongUniversity，Shanghai200030，China； 3．ShanghaiKeyLabofVeterinaryBiotechnology，Shanghai200240，China； 4．ShanghaiXiangxinLivestockPoultryCo．Ltd．，Shanghai201302，China) Abstract：Theaim ofthisworkwastotrytodiscoverpolymorphismsandstudytheeffectsoftheswine genesVEGlFA ，H F1A andEPAS1on reproductivetraits．Afteralignmentofsequencesofdifferent individuals，27potentialSNPsweredetected．Four(g．1438C~G，g．1811CG，g．6973G~ T andg．12532C T)oftheseSNPscanbedetectedbyPCR_RFLPusingdigestionofthePCR fragmentwithB5H2工， PspEI，Eco32I andBcn工 in theintronin theporcineVEGFA gene．TheotherSNPscannotbe recognizedbyPCR—RFLP．TheallelesoffourSNPswere distributedwith obviousdifference in allele frequencybetweenMeishan pig and French LargeWhitepig．Theg．6973G T polymorphism ofthe VEGFA geneshowedsignificantcorrelationwithTNB andNBA inlaterparities(P O．05)inMeishan pigs．Ten、／=GFc4 haplotypes (GTCT，CGCC，GTGC，CGCT，CGCC，GGGC，GGCT，GGGT，GTCC and CTGT)weredetectedinMeishanpigs，whileonlytwohaplotypes(GTGC andGTCT)inFrenchLarge Whitepigs．TheresultsshowedthatGGGChaplotypehadignificanteffectsonTNB／NBA insecondand subsequentlitters(P0．05)．GTGC haplotypehadasignificanteffectOnTNBinfir