黑老虎的化学成分的研究.docVIP

黑老虎的化学成分研究 王楠，李占林，华会明 （沈阳药科大学中药学院，辽宁，沈阳 110016） 摘要：目的 对黑老虎（Kadsura coccinea）的化学成分进行研究。方法 利用多种色谱方法进行成分分离，根据物理化学性质和波谱学手段对分离得到的化合物进行结构鉴定。结果 从黑老虎中分离得到7个新的羊毛脂烷型三萜类化合物，分别鉴定为23-oxo-3,4-seco-9βH-lanost-4(28),7-dien-3-oic acid（1）、23-oxo-3,4-seco-9βH-lanost-4(28),7,24- trien-3-oic acid（2）、25-hydroxy-23-oxo- 3,4-seco-9βH-lanost-4(28),7-dien-3-oic acid（3）、25-hydroxy-3,4-seco-9βH-lanost-4(28),7,23-trien-3-oic acid（4）、24R-hydroxy-3,4-seco-9βH- lanost-4(28),7,25(26)-trien-3-oic acid（5）、24(E)-3,4-seco-8βH-lanost-4(28),9(11),24-trien-3,26- dioic acid（6）和23-oxo-8βH-lanost-9(11)-ene-3,4-olide（7）。结论 化合物1，2，3和5具有抑制白血病HL-60细胞增殖作用，其中以化合物1抑制细胞增殖的作用最强。 关键词：黑老虎；化学成分；结构鉴定 Studies on chemical constituents of Kadsura coccinea Nan-wang, Zhan Lin-li, Hui Ming-hua* (School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China) ABSTRACT: OBJECTIVE To study the chemical constituents of the roots of Kadsura coccinea. METHODS The compounds were isolated by chromatography on silica gel. Their structures were identified by their physical and spectral data. RESULTS Seven compounds were isolated and identified as 23-oxo-3,4-seco-9βH-lanost-4(28),7-dien-3-oic acid (1), 23-oxo- 3,4-seco-9βH-lanost-4(28),7,24-trien-3-oic acid (2), 25-hydroxy-23-oxo-3,4-seco-9βH-lanost- 4(28),7-dien-3-oic acid (3), 25-hydroxy-3,4-seco-9βH-lanost-4(28),7,23-trien-3-oic acid (4), 24R- hydroxy-3,4-seco-9βH-lanost-4(28),7,25(26)-trien-3-oic acid (5), 24(E)-3,4-seco-8βH-lanost- 4(28),9(11),24-trien-3,26-dioic acid (6) and 23-oxo-8βH-lanost-9(11)-ene-3,4-olide (7). CONCLUSION Compounds 1, 2, 3, and 5 showed inhibitory activity against HL-60 human leukemia cells, while compound 1 was the most effective antiproliferative agent. KEY WORDS: Kadsura coccinea; chemical constituents, structure identification 黑老虎为五味子科南五味子属植物Kadsura coccinea (Lem.)A.C.Sm.的根及藤茎[1]。广泛分布于我国广东、广西、海南、贵州、四川等华南地区和西南地区。其性温，味辛、微苦，具有行气止痛、祛风活络、散瘀消肿之功效。黑老虎在民间常用于治疗胃、十二指肠溃疡、慢性胃炎、急性胃肠炎、风湿性关节炎、跌打肿痛、痛经、产后瘀血腹痛等症[2]。广西中医药研究所