Overexpression+of+brain-derived+neurotrophic+factor+in+the+hippocampus+protects+against+post-stroke+depression.pdf

NEURAL REGENERATION RESEARCH September 2015,Volume 10,Issue 9 RESEARCH ARTICLE Overexpression of brain-derived neurotrophic factor in the hippocampus protects against post-stroke depression 1 1 2 2 3 3 1 1, * Hao-hao Chen , Ning Zhang , Wei-yun Li , Ma-rong Fang , Hui Zhang , Yuan-shu Fang , Ming-xing Ding , Xiao-yan Fu 1 Medical Molecular Biology Laboratory, School of Medicine, Jinhua Polytechnic, Jinhua, Zhejiang Province, China 2 Institute of Neuroscience, School of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China 3 Center of Experimental Animals, Jinhua Food and Drug Administration, Jinhua, Zhejiang Province, China Abstract *Correspondence to: Post-stroke depression is associated with reduced expression of brain-derived neurotrophic Xiao-yan Fu, M.D., fxythd@. factor (BDNF). In this study, we evaluated whether BDNF overexpression affects depression-like behavior in a rat model of post-stroke depression. The middle cerebral artery was occluded to orcid: produce a model of focal cerebral ischemia. These rats were then subjected to isolation-housing 0000-0001-5496-7944 (Xiao-yan Fu) combined with chronic unpredictable mild stress to generate a model of post-stroke depression. A BDNF gene lentiviral vector was injected into the hippocampus. At 7 days after injection, doi: 10.4103/1673-5374.165510 western blot assay and real-time quantitative PCR revealed that BDNF expression in the hippo- /