In vivo metabolism of apolipoprotein S in humans. Comparison with apolipoprotein A-I metabolism》.pdfVIP

In vivo metabolism of apolipoprotein S in humans. Comparison with apolipoprotein A-I metabolism》.pdf

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In vivo metabolism of apolipoprotein S in humans. Comparison with apolipoprotein A-I metabolism》.pdf

Clink Chimica Acta, 170 (1987) 169-180 169 Elsevier CCA 03981 In vivo metabolism of apolipoprotein S in humans. Comparison with apolipoprotein A-I metabolism Claude L. Malmendier, Jean-Fraqois Lontie and Claude Delcroix Research Foundation on Atherosclerosis, and Research Unit on A therosclerosrs, Faculiy OJMedicine, Uniuersith Libre de Bruxelles, Brussels (Belgam) (Received 26 March 1987; revision received 1 July 1987; accepted after revision 3 August 1987) Key words: Apohpoprotein A-I; Apolipoprotein S; Metabolism; Normolipoproteinemia; Plasma-Urine; Compartmental modeling Summary 125I-labelled apolipoprotein (APO) S and ‘311-labelled apolipoprotein A-I were injected i.v. into healthy volunteers. Blood samples and daily urine collections were drawn periodically for 15 days. Ninety-eight percent of r3iI radioactivity and 95% of 125I radioactivity were found in HDL after Superose gel chromatography of plasma. About 10% of each radioactivity was recovered in the d 1.250 infranate after one ultracentrifugation. Affinity chromatography on monoclonal anti-APO A-I Sepharose column separates two lipoprotein particles containing Apo S, one re- tained with Apo A-I (42.5%) and the other eluting without Apo A-I (57.5%). Kinetic parameters were calculated according to exponenti

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