In vivo metabolism of apolipoprotein S in humans. Comparison with apolipoprotein A-I metabolism》.pdfVIP
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In vivo metabolism of apolipoprotein S in humans. Comparison with apolipoprotein A-I metabolism》.pdf
Clink Chimica Acta, 170 (1987) 169-180 169
Elsevier
CCA 03981
In vivo metabolism of apolipoprotein S in humans.
Comparison with apolipoprotein A-I metabolism
Claude L. Malmendier, Jean-Fraqois Lontie and Claude Delcroix
Research Foundation on Atherosclerosis, and Research Unit on A therosclerosrs, Faculiy OJMedicine,
Uniuersith Libre de Bruxelles, Brussels (Belgam)
(Received 26 March 1987; revision received 1 July 1987; accepted after revision 3 August 1987)
Key words: Apohpoprotein A-I; Apolipoprotein S; Metabolism; Normolipoproteinemia; Plasma-Urine;
Compartmental modeling
Summary
125I-labelled apolipoprotein (APO) S and ‘311-labelled apolipoprotein A-I were
injected i.v. into healthy volunteers. Blood samples and daily urine collections were
drawn periodically for 15 days. Ninety-eight percent of r3iI radioactivity and 95%
of 125I radioactivity were found in HDL after Superose gel chromatography of
plasma. About 10% of each radioactivity was recovered in the d 1.250 infranate
after one ultracentrifugation. Affinity chromatography on monoclonal anti-APO A-I
Sepharose column separates two lipoprotein particles containing Apo S, one re-
tained with Apo A-I (42.5%) and the other eluting without Apo A-I (57.5%).
Kinetic parameters were calculated according to exponenti
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