Protein kinase CK2 is necessary for the adipogenic differentiation of human mesenchymal stem cells》.pdf

Biochimica et Biophysica Acta 1853 (2015) 2207–2216 Contents lists available at ScienceDirect Biochimica et Biophysica Acta jour nal homepage: www.e lsevie r. com/ locate/ bbamcr Protein kinase CK2 is necessary for the adipogenic differentiation of human mesenchymal stem cells Lisa Schwind a,1, Nadine Wilhelm a,b,1, Sabine Kartarius a, Mathias Montenarh a, Erwin Gorjup b, Claudia Götz a,⁎ a Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, Geb. 44, D-66421 Homburg, Germany b Fraunhofer-Institut für Biomedizinische Technik IBMT, Ensheimer, Strasse 48, D-66386 St. Ingbert, Germany a r t i c l e i n f o a b s t r a c t Article history: CK2 is a serine/threonine protein kinase, which is so important for many aspects of cellular regulation that life Received 13 November 2014 without CK2 is impossible. Here, we analysed CK2 during adipogenic differentiation of human mesenchymal Received in revised form 21 May 2015 stem cells (hMSCs). With progress of the differentiation CK2 protein level and the kinase activity decreased. Accepted 23 May 2015 Whereas CK2α remained in the nucleus during differentiation, the localization of CK2β showed a dynamic shuttling Available online 27 May 2015 in the course of differentiation. Over the last years a large number of inhibitors of CK2 kinase activity were generated Keywords: with the idea to use them in cancer therapy. Our results show that two highly specific i