Proteomic investigation of Sri Lankan hump-nosed pit viper (Hypnale hypnale) venom》.pdf

Toxicon 93 (2015) 164e170 Contents lists available at ScienceDirect Toxicon journal homepage: /l ocate/toxicon Proteomic investigation of Sri Lankan hump-nosed pit viper (Hypnale hypnale) venom Choo Hock Tan a, c, *, Nget Hong Tan b, c, Si Mui Sim a, Shin Yee Fung b, c, Christeine Ariaranee Gnanathasan d a Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia b Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia c University of Malaya Proteomic Centre for Research (UMPCR), University of Malaya, Kuala Lumpur, Malaysia d Department of Clinical Medicine, Faculty of Medicine, University of Colombo, Sri Lanka a r t i c l e i n f o a b s t r a c t Article history: The hump-nosed pit viper, Hypanle hypnale, contributes to snakebite mortality and morbidity in Sri Received 19 July 2014 Lanka. Studies showed that the venom is hemotoxic and nephrotoxic, with some biochemical and Received in revised form antigenic properties similar to the venom of Calloselasma rhodostoma (Malayan pit viper). To further 18 November 2014 characterize the complexity composition of the venom, we investigated the proteome of a pooled venom Accepted 20 November 2014 Available online 21 November 2014 sample from 10 Sri Lankan H. hypnale with reverse-phase high performance liquid chromatography (rp- HPLC), sodium dodecyl sulfate-polyacryla