Purification, characterization and immunoreactivity of β′-component, a major allergen from the roe of large yellow croaker (Pseudosciaena crocea)》.pdf

Food and Chemical Toxicology 72 (2014) 111–121 Contents lists available at ScienceDirect Food and Chemical Toxicology journal homepage: www. /loc ate/foodchemtox 0 Purification, characterization and immunoreactivity of b -component, a major allergen from the roe of large yellow croaker (Pseudosciaena crocea) Yan-Yun Liu a, Min-Jie Cao a, Ming-Li Zhang b, Jia-Wei Hu a, Yong-Xia Zhang a, Ling-Jing Zhang a, Guang-Ming Liu a,⇑ a College of Food and Biological Engineering, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Jimei University, 43 Yindou Road, Xiamen, Fujian 361021, China b Xiamen Second Hospital, 566 Shengguang Road, Xiamen, Fujian 361021, China a r t i c l e i n f o a b s t r a c t Article history: Fish roe, a nutritious food, is favored by consumers, but has also been confirmed to be allergenic in Received 18 April 2014 salmonid fish. However, little information is available in other fish species. To determine the allergen Accepted 8 July 2014 in the roe of large yellow croaker (Pseudosciaena crocea), crude extracts were incubated with sera of Available online 18 July 2014 allergic patients. The major allergen was purified by column chromatography methods, revealing a single 0