Quantification of apolipoprotein A-I mimetic peptide D-4F in rabbit plasma by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry》.pdf

Journal of Chromatography B, 877 (2009) 1946–1950 Contents lists available at ScienceDirect Journal of Chromatography B j o u rn al h omepage: w w w. elsev /locate/chro mb Short communication Quantification of apolipoprotein A-I mimetic peptide D-4F in rabbit plasma by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry Xiaoyi Hu ∗ , Marika Kamberi Abbott Laboratories, Santa Clara, CA 95054, United States a r t i c l e i n f o a b s t r a c t Article history: The apolipoprotein A-I mimetic peptide D-4F is a potential therapeutical agent effective in maintaining Received 9 February 2009 cardiovascular health. A bioanalytical assay based on high-performance liquid chromatography coupled Accepted 29 April 2009 with electrospray ionization tandem mass spectrometry (LC/MS/MS) to quantitate the D-4F amount in Available online 13 May 2009 rabbit plasma was developed and validated. A compound with a close structure similarity to the D-4F (only one amino acid A–V altered) was used as an internal standard. Both D-4F and the internal standard were Keywords: extracted by protein precipitation using acetonitrile/0.2% Triton XL 80N. The correlation coefficient of the Apolipoprotein A-I mimetic peptide calibration curve was 0.9991 in the range 20–40,000 ng/mL. This assay can be used for pharmacokinetic D-4F Quantification studies of the drug. Also, it may be adjusted for the quantification of other