Real time loop-mediated isothermal amplification using a portable fluorescence scanner for rapid and simple detection of Vibrio parahaemolyticus》.pdf

Food Control 41 (2014) 91e95 Contents lists available at ScienceDirect Food Control journal homepage: /locate/foodcont Real time loop-mediated isothermal amplification using a portable fluorescence scanner for rapid and simple detection of Vibrio parahaemolyticus MinYing Yi a, Li Ling a, Sucharit B. Neogi c, d, YaoSen Fan b, DaYun Tang b, Shinji Yamasaki c, Lei Shi b, e, Lei Ye b, e, * a Guangdong Inspection and Quarantine Technology Center, Microbiological Test Department of Food Testing Lab, 510623 Guangzhou, PR China b College of Light Industry and Food Sciences, South China University of Technology, 510640 Guangzhou, PR China c Graduate School of Life and Environment Sciences, Osaka Prefecture University, Osaka 598-8531, Japan d International Centre for Diarrhoeal Diseases Research, Mohakhali, Dhaka 1212, Bangladesh e Guangzhou DE AOU Biotechnology Technology Co., Ltd, Innovation Building, Science Road, Guangzhou Science City, 510663 Guangzhou, PR China a r t i c l e i n f o a b s t r a c t Article history: We evaluated the usefulness of the loop-mediated isothermal amplification (LAMP) using a portable ESE Received 30 May 2013 Quant tube scanner as a rapid and simple method for the detection of Vibrio parahaemolyticus, an Received in revised form important pathogen causing seafood-borne gastroenteritis. The real time LAMP (RT-LAMP) assay using a 26 December 2013 hemolysin gene (tlh/ldh)-specific primers was verified using V. parahaemolyticus strains (n ¼ 91) from Accepted 4 January