发根农杆菌抑菌剂的抑菌效果及对茶组培苗丛生芽的影响.pdf

茶叶科学 20 15，35 （5 ）：437~442 Journal of Tea Science 投稿平台： 发根农杆菌抑菌剂的抑菌效果及对茶组培苗丛生芽的影响 杨亚萍，李永兰，梁月荣，陆建良，郑新强* 浙江大学茶叶研究所，浙江 杭州 310058 摘要：研究了 MS 培养基中不同浓度头孢噻肟钠 （Cefotaxime sodium ）、羧苄青霉素（Carbenicillin ）和特美 汀（Timentin ）3 种抑菌剂对发根农杆菌的抑制效果及对茶树组培苗丛生芽的影响。结果显示，头孢噻肟钠和 特美汀均可以有效抑制农杆菌 LBA9402 、15834，而 15834 在羧苄青霉素的板上 17 d 内长出；对于外植体，头 孢噻肟钠和羧苄青霉素都显著降低了茶组培苗的丛生芽增殖率，且畸形苗率高；当特美汀质量浓度在 400 mg ·L- 1 以下时，对茶组培苗影响较小，与正常对照无显著差异，特美汀具有作为茶树遗传转化体系中抑菌剂的 潜力。 关键词：茶树；农杆菌；抑菌剂；生理 中图分类号：S571.1 ；Q939.1 文献标识码：A 文章编号：1000-369X （2015 ）05-437-06 Antibiotics Inhibition to Agrobaceterium Rhizogenes and Effect to Tea Multiple Shoots * YANG Yaping, LI Yonglan, LIANG Yuerong, LU Jianliang, ZHENG Xinqiang Zhejiang University Tea Research Institute, Hangzhou 310058 , China Abstract: Effects of three antibiotics on inhibition of Agrobacterium Rhizogenes LBA4404 and 15834, bud formation and growth of tea plant were studied. The results sh owed that cefotaxime sodium and timentin can completely inhibit the growth of Agrobacterium LBA9402 and 15834, however, carbenicillin only inhibit LBA9402 bacterium but not 15834 bacterium . Bud regeneration and growth are significantly inhibited by cefotaxime sodium and carbenicillin treatment . There is no significant difference between Timentin ( ≤400 mg ·L- 1) treatment and control without antibiotics on bud regeneration and growth of tea cultur e seedlings. Timentin is a good alternative antibiotic to eliminate Agrobacterium tumefaciens in tea plant genetic transformation system. Keywords: Camellia sinensis, Agrobacterium , antibiotic, physiology 在农杆菌介导的植物基因转化中，外植体 是头孢霉素类。300 mg ·L- 1 以