心肺体外循环后大鼠长期空间认知功能损害和海马区微胶质细胞激活.pdf

中国临床神经科学 2015 年第 23 卷第1 期 Chin J Clin Neurosci 2015, 23 (4), 361~365Chin J Clin Neurosci 2015, 23 (3), ·361· 论 著 1 2 3 吴瀚峰 ，张 钰 ，张法永 1 2 3 ( 上海伽玛医院神经外科 200235 ；加州大学圣地亚哥分校生物化学系细胞生物学专业92093；复旦大学 附属华山医院神经外科200040) 关键词　心肺体外循环；认知功能障碍；微胶质细胞；炎性反应 摘要 目的　探讨心肺体外循环(CPB) 对大鼠长期空间认知功能和脑内炎性反应的影响。方法　雄性成年SD 大鼠随 机分为对照组、假手术组和CPB 组，每组6 只。在CPB 术后20 周，采用8- 臂迷宫实验测定大鼠的空间认知功能， 应用免疫组化学检测海马区微胶质细胞的活化情况。结果　与对照组和假手术组比较，CPB 组呈现明显的学习和记 忆能力损害。CPB 组训练所需的次数较其他两组显著增加；在记忆测试中，间隔3 h 和6 h 后，CPB 组记忆准确率 明显低于其他两组(P0.05) 。免疫组化学检测示CPB 组海马区有活化的微胶质细胞。结论　CPB 可引起长期认知功 能损害，这些损害可能与CPB 引起海马区微胶质细胞活化有关。 The Effect and Mechanism of Experimental Cardiopulmonary Bypass on Long-term Cognitive 1 2 3 1Department of Neurosurgery, Impairment in Rats WU Han-feng , ZHANG Yu , ZHANG Fa-yong 　 Shanghai Gamma Hospital, Shanghai 200235, China; 2Department of Biochemistry/Cell Biology Division of Biological Science, University of California San Diego, CA 92093, USA; 3Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, China KEY WORDS cardiopulmonary bypass; cognitive impairment; microglia; inflammatory reaction ABSTRACT Aim To observe the effect of experimental cardiopulmonary bypass (CPB) on persistent cognitive impairment and cerebral inflammatory reaction in rats. Methods The adult male Sprague-Dawley rats were randomly assigned to control, sham, and CPB groups. Twenty weeks later, cognitive function was tested with 8-arm maze. Immunohistochemical study was used to detect the activation of microglia. Results The ability of learning and memory was impaired in CPB rats compared with that of the sham and the