小麦Stpk-Ⅴ同源基因的克隆及全蚀菌诱导下的表达特征分析.pdf

植物病理学报 ACTA PHYTOPATHOLOGICASINICA 45(4)：443—448(2015) doi：10．13926／j．cnki．apps．2015．04．014 小麦Stpk—V同源基因的克隆及全蚀菌诱导下 的表达特征分析 王俊美，徐飞，杨共强，宋玉立4，李亚红，田怀芹 (河南省农业科学院植物保护研究所／农业部华北南部作物有害生物综合治理重点实验室，郑州450002) and of underinfection CloningexpressionanalysisStpk—V homologousgenes by var。triticiinwheatWANG Gaeumannomycesgraminis Jun—mei，XUFei，YANG Gong— ofPlant of qiang，SONGYu—li，LIYa—hong，TIANHuai—qin(Institute Protection，Henan AcademyAgricultural of ofthe Pest SouthemofNorth of ofthe Sciences；KeyLaboratoryCropIntegratedManagement China，Ministry Agriculture of 450002，China) People’SRepublicChina，Zhengzhou Abstract：Take-alldisease，caused var．triticiisoneofthe soil-borne byGaeumannomycesgraminis important diseasesonwheat．Theresistanceresearchestotake—allhavebeen genes focusedontheirfunctionalin study resistant this havebeenclonedwiththe breeding．Instudy，theStpk—Vhomologousgenes primerssynthesized totheknown of accession number thesetwo according sequenceStpk—Vgene(GenBank HM241655．1)，and Stpk—V wereisolatedfromtwowheatcultivars an