小麦粒重基因TaGW2-6A等位变异的组成分析及育种选择.pdf

作物学报 ACTA AGRONOMICA SINICA 2015, 41(11): 16401647 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2015.01640 小麦粒重基因TaGW2-6A 等位变异的组成分析及育种选择 寇 程 高 欣 李立群 李 扬 王中华 李学军* / , 712100 : 6A TaGW2 , 8 T , Hap-6AA Hap-6AG (high resolution melting curve analysis, HRM)Hap-6A-P1/P2 316 () TaGW2-6A 2 , , 22 , TaGW2-6A 61 T (977T )255 T (977) ; 977T , Hap-6AA (TA)Hap-6AG (TG)29 32 , 977, Hap-6AA (A)Hap-6AG (G) 160 95 , 977T 977(P0.05)(P0.001) (P0.001), Hap-6AA Hap-6AG (P0.05)(P0.05)(P0.001)TaGW2-6A , , TA TGAG , 22 TA , , 8425B, , TA , TaGW2-6A TA : ; TaGW2 ; ; (HRM); Composition and Selection of TaGW2-6A Alleles for Wheat Kernel Weight * KOU Cheng, GAO Xin, LI Li-Qun, LI Yang, WANG Zhong-Hua, and LI Xue-Jun College of Agronomy, Northwest AF University / State Key Laboratory of Crop Stress Biology in Arid Areas, Yangling 712100, China Abstract: TaGW2 on chromosome 6A is a key gene governing kernel size of wheat (Triticum aestivum L.). There are a single T-base insertion in the eighth exon of TaGW2 (977 bp) and two SNPs (Hap-6AA and Hap-6AG) in the promoter region. In this study, 316 wheat varieties (lines) were detected by high resolution melting curve (HRM) analysis and Hap-6A-P1/P2 molecular marker for TaGW2-6A allelic variations and their correlations with kernel length, kernel wi