《Oxidative-damage effect of Fe3O4 nanoparticles on mouse hepatic and brain cells in vivo.》.pdf

Front．Bio1．2013，8(5)：549555 D0I10．1007／sl1515-013．1277—8 Oxidative-damageeffectofFe304nanopa iclesonmouse hhqeppaattiiccaanndbrraaiinncceellssinvivvoo YongliWANG ，NianQIN ，ShanCHEN，JingyunZHAO，XuYANG(函) HubeiKeyLaboratoryOiGeneticRegulationandIntegrativeBiologyCollegeoilfieScience,CentralChinaNormalUniversity,Wuhan 430079,China ◎ HigherEducationPressandSpringer-VerlagBerlinHeidelberg2013 Abstract ToassessthebiologicalsafetyofFe3O4nanoparticles Ps)，theoxidative—damageeffectoftheseNPswas studied．Twenty．fiveKunmingmicewereexposedtoFe3O4NPsbyintl’aperitonealinjectiondailyfor1weekatdosesof 0，10，20，and40mgk·g ．FiveKunmingmicewerealsoinjectedwith40mgk·g ordinaryFe304particlesunderthe samephysiologicaleonditions．Biomarkersofreactiveoxygenspecies(ROS)，glutathione(GSH)，andmalondialdehyde rMDA)inthehepaticandbraintissuesweredetected．Resultsshowedthatnosignificantdifferenceinoxidativedamage existedatc0ncentratiOnslowerthan10mg·kg ofrNPscomparedwiththecontrolgroup．Fea}04NPconcentrationhad obviousdose-effectrelationships ’0．050rP0．01)withROSlevel，GSHcontent，andMDAcontentinmousehepatic and brain tissues at20mg·kg concentrations．To some extent，ordinary Fe3O4 particleswith 40m g·kg concentration also affected hepaticand brain tissuesin mice．Thebiologicaleffectwassimilarto Fe3O4NPsat 10mg·kg～ concentration．Thus，Fe3o4NPshadsignificantdamageeffectsontheantioxidantdefensesystem inthe hepaticandbraintissuesofmice，whereasordinary Fe304hadlessinfluencethanFe3O4NPsatthesameconcentration． Keywords Fe3O4nanoparticle(NP)，ordinaryFe304particle，oxidativedamage，reactiveoxygenspecies(ROS)，glutathione (GSH)，malondialdehyde(MDA) Introduction toa~ach interestedcells．In theprogressoftheinteraction bewt eenNPsandcells．ensuringthesafetyofanorganism is Nanoparticles Ps1arespecialmaterialswithtwoorthree veryimportant(Lewinskietal一2008)． dimensionsranging f