《高效降解纤维素菌系的筛选分离及复合菌系的构建（英文版）》.pdfVIP

AgricuIturaI Science TechnoIogy, 2015, 16(4): 639-644, 652 Copyright 訫 2015, Information Institute of HAAS. AII rights reserved MoIecuIar BioIogy and Tissue CuIture Screening and lsolation of Highly Efficient Cellulose-degrading Microorganisms and Construction of Complex Microbial System 1 2 1 * Tingting SHANG , Quanhong Ll , Shanggui DENG 1. CoIIege of Food and PharmacoIogy, Zhejiang Ocean University, Zhoushan 316022, China; 2. CoIIege of Food Science and NutritionaI Engineering, China AgricuIturaI University, Beijing 100083, China Abstract This study aimed to screen a highIy efficient ceIIuIose-degrading compIex 高效降解纤维素菌系的筛选分 microbiaI system from soiI and rotten straw and then study its appIication to naturaI 离及复合菌系的构建 ceIIuIose. The isoIated strains were preIiminariIy screened with Congo red staining and the ceIIuIases activities were determined with DNS method. The non-antagonis- 尚婷婷 1 ，李全宏 2 ，邓尚贵 1* （1. 浙江海洋学 tic highIy efficient ceIIuIos-degrading strains were seIected and cuItured combinedIy 院食品与 医药 学院 ，浙江 舟山 316022 ；2. 中 国 for deveIoping a ceIIuIose-degrading compIex microbiaI system. The resuIts showed 农 业 大 学 食 品 科 学 与 营 养 工 程 学 院 ， 北 京 that the CMC enzyme activity of the mixed cuIture of the three fungi strains was 100083） higher than those of the cuItures of the three singIe strains. The morphoIogicaI and moIecuIar bioIogicaI identification indicated that F1 was Botryosphaeria, F2 was Rhi- 摘 要 为了从土壤及腐烂的秸 秆中 筛选一 zopus oryzae, and F5 was Fusarium oxysporum. When straw was used as carbon 组高效降解纤维素的复合菌系 ，并研究其在天 source, the CMC enzyme activities