医用臭氧介入治疗腰椎间盘突出症对其自身免疫作用影响的临床的研究.pdfVIP

内 容 摘 要 目的：1.观察应用医用臭氧介入治疗腰椎间盘突出症患者血清中IgG、IgM 、 TNF-α、IL-6、IL-10 、IL-12 水平的变化。2.探讨腰椎间盘突出症的自身免疫机制和医 用臭氧介入治疗腰椎间盘突出症可能的免疫作用机理。 方法：以2006.6-2008.8 葛洲坝中心医院行医用臭氧介入治疗腰椎间盘突出症60 例患者为研究对象，设为实验组。60 例健康成年人设为对照组。运用免疫透射比浊 法测定实验组术前、术后三天、术后一月和对照组血清IgG、IgM 的含量。运用酶联 免疫吸附法测定实验组术前、术后三天、术后一月和对照组血清TNF-α、IL-6、IL-10、 IL-12 的含量。 结果：与对照组比较，实验组术前血清IL-6、IL-10 、IL-12、TNF-α、IgG、IgM 水平均升高（p0.01 ）。与实验组术前比较，实验组术后IL-12 、TNF-α、IgG、IgM 水 平均降低（p0.01 ）。与实验组术前比较，实验组术后血清IL-6、IL-10 水平均升高 （p0.01 ）。与术后三天比较，实验组术后一月IL-12 、TNF-α、IgG、IgM 水平均降低 （p0.05 ）。与术后三天比较，实验组术后一月IL-10 水平升高（p0.05 ）。与术后三 天比较，实验组术后一月IL-6 水平变化不大（p ﹥0.05 ）。 结论： 1.腰椎间盘突出症患者机体存在自身免疫状态的异常改变，表现在细胞 免疫和体液免疫两个方面。2.医用臭氧在腰椎间盘突出症的介入治疗中发挥了免疫抑 制作用。3.抑制自身免疫反应是医用臭氧介入治疗腰椎间盘突出症重要机制之一。 关键词：腰椎间盘突出症 医用臭氧 自身免疫反应 II Abstract Objective: 1. To observed the changes of serum IgG 、IgM 、TNF-α、IL-6 、IL-10 and IL-12 levels of patients with Lumbar disc herniation with medical ozone injection ．2. To evaluate the mechanism of the autoimmune response of patients with Lumbar disc herniation with medical ozone ．To investigate immune mechanism of patients with Lumbar disc herniation with medical ozone ． Method ： Sixty patients with Lumbar disc herniation with medical ozone injection from June 2006 to August 2008 were set as testing group ．Sixty Healthy adults were set as control group ．At the pretreatment 、three days and one month after treatment ，The serum IgG and IgM levels were detectd by immunoturbidimetry method ．At the pretreatment 、three days and one month after treatment ，The serum TNF-α、IL-6、IL-10 and IL-12 levels were detectd by enzyme linked immunosorbent assay ． Result: The serum IL-6、IL-10 、IL-12、TNF-α、IgG、IgM levels in testing group before treatment were increased compared with the control group （p0.01 ）；The serum IL-12、 TNF-α、IgG、IgM levels in testing group were decr