《Digestive enzyme activity and mRNA level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus》.pdfVIP
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《Digestive enzyme activity and mRNA level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus》.pdf
ChineseJournalof and
OceanologyLimnology
V01.26No.1.P.62—68.2008
andmRNAlevel in
activity of
Digestiveenzyme trypsin
redclaw
crayfish,Chetaxquadricarnatus术
embryonic
LUO
Wen(罗文)t,tt Zhongliang(周忠良)”,
ZHA..OYunlong(赵云握)什·”,ZHOU
AN
Chuanguang(安传光)”,MAQiang(马强)”
Artsand
,SchoolofLi#Science,CollegeofShaoxingSciences.Shaoxing312000,China)
ChinaNormal 200062。China)
,SchooloILifeScience,East University,Shanghai
Received Jan.1
12.2006;revision 8,2007
May accepted
AbstractT11e andmRNAlevelof the
enzyme trypsinduring
digestive activity embryonic
ofCh£,乜rd“(删ricarinatuswere biochemicaIand
development analyzedusing FluorogenicQuantitative
resultsshowthattheactivitiesof and hadtwo
PCR(FO—PCR)methods.The trypsinchymotrypsin
different increasedinthe of
specificactivity rapidly earlystagesdevelopment
changepatterns.Trypsin
andstillremainedin forthehatch in
highpreparation stage.However,chymotr3‘psinactivitypeakedstage
iu mRNA
4of anddecreased thelast levelof was
embryonicdevelopment significantlystage.The trypsin
elevatedinall andtwo valueswereobservedin 2and5 results
stages peak stages respectively.The
indicatethat is fortheutilizationofthe and
trypsinveryimportant yolkduringembryonicdevelopment
forthe
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