《几株硅酸盐细菌菌株的分离及解钾、解硅活性 Isolation of Several Silicate Bacteria Strains and Determination of Their Activity of Releasing Silicon and Potassium》.pdfVIP

第52卷第21期 湖北农业科学 V01．52No．21 Sciences Nov．，2013 2013年11月Hubei Agricultural 几株硅酸盐细菌菌株的分离及解钾、解硅活性 李 佳，张爱民，王 伟，朱宝成 河北农业大学生命科学学院，河北保定071001 摘要：从水稻田和玉米田土样中分别分离适用于水田作物和旱田作物的硅酸盐细菌，对能够在亚历山大 罗夫培养基上生长且符合硅酸盐细菌茵落特征的细菌茵株进行茵种鉴定，然后浸13定在不同供氧条件下 菌株的解硅、解钾能力。结果表明。从水稻田土样中分离得到菌株2株，从玉米田土样中分离得到菌株l 株．经鉴定均属于硅酸盐细菌的一种——胶冻样类芽孢杆菌 Paenibacillusmucilaginosus 。在不同供氧条 件下．这3株菌株均具有一定的解硅、解钾活性，供氧充足的条件下菌株h一5的解硅活性最高，可以使发 酵液中水溶性硅含量达到4．5恤∥hL以上，菌株s4解钾活性最高，可以使水溶性钾含量达到46．73mg／L 以上．分离自水田的2株菌株在微氧条件下仍能保持较高的解硅、解钾活性，具有作为生物肥料的出发 菌株应用于水田作物的较大潜力。 关键词：硅酸盐细菌；分离；鉴定；解硅活性；解钾活性 中图分类号：S154．3 文献标识码：A 文章编号：0439—8114 2013 21—5147—06 SilicateBacteriaStrainsandDeterminationofTheir of IsolationofSeveral Activity SiliconandPotassium Releasing LI Jia，ZHANGAi-min，WANGWei，ZHU Bao—cheng ofLife 071001，Hebei，China College Science，HebeiA画culturalUniversity，Baoding soil of fieldinHunanareaandcornfieldinHebei silicatebacteriastrains Abstract：Fromthe samplespaddy area，several of sili- wereisolatedThenthestrainswereidentified andculturefeaturesobservation．The activity bymorphological releasing conand ofthesestrainson levelsof dissolutionweredetermined．Theresultsshowedthattwo potassium different oxygen soil threestrainswereall strainsandonestrainwereobtainedfrom andcornfield identified paddy soil．respectively．These asPaenibacillus kindofsilicatebacteria．Thesethreestrainshada certainlevelof of sili- mucilaginosoz，a activityreleasing conand h-5hadthe of contentofwatersolublesilicon them，strain silicon，the potassium．Among highestactivityreleasing initsfermentationbrothcouldreachover4．5 s-4hadthe of content Ixg／mL．Strain highestactivityreleasingpotassium．the ofwatersoluble initsbrothwereover46．73 of siliconand oftwostrains potassium m#L．Theactivityreleasing potassium obtained soilwerestabler levelsof that strain．Onthe of from ondifferent dissolutionthan ofother condition paddy oxygen low two obtainedfrom soilcouldstill a of silicon dissolution，thestraina oxygen paddy keeprelativelyhighactivityreleasing and pota