不同中医证型骨髓增生异常综合征RIZ1、ID4基因甲基化异常表达及临床意义.pdfVIP

Abnormal expression and significance of RIZ1 and ID4 gene methylation in myelodysplastic syndromes with different TCM syndromes Special: Internal medicine of TCM Student: Xuan Lina Tutor: Prof. Xu Ruirong Abstract Objective To investigate the methylation status of RIZ1 and ID4 gene promoter in patients with different TCM syndromes of myelodysplastic syndromes (MDS), and to study the relationship of RIZ1 and ID4 gene methylation in different TCM syndromes of MDS. Method The prognostic scores for 35 patients were calculated using the International Prognostic Scoring System (IPSS), the methylation status of RIZ1 and ID4 gene promoter in bone marrow mononuclear cells of 35 MDS patients and 15 patients with benign hematological diseases was detected by methylation specific polymerase chain reaction (MS-PCR). Result (1) There was a significant correlation between traditional Chinese medical syndromes and IPSS prognostic scores (P0.001, R=0.596). Patients with stasis toxin prevalence had worse prognosis than patients with spleen and kidney deficiency. (2) The RIZ1 and ID4 genes were all completely unmethylated in bone marrow samples from patients with benign hematological diseases as control. RIZ1 and ID4 gene methylation was found in 15 and 12 cases of 35 MDS respectively, which were higher than that in the control group (P=0.006 vs P=0.01). (3) Different degrees of RIZ1 gene methylation was found in different types of MDS, but there were no significant differences amang them. (4) The positive rate of ID4 gene in patients with stasis toxin prevalence was much higher than that with vital qi deficiency, there was significant difference between the two group (P=0.011). Patients with marrow blast ratio beyon