《Analysis and validation of genome-specific DNA variations in 5#39; flanking conserved sequences of wheat low-molecular-weight glutenin subunit genes》.pdfVIP

322 Science in China Series C: Life Sciences 2006 Vol.49 No.4 322 331 DOI: 10.1007/s11427-006-2007-0 Analysis and validation of genome-specific DNA variations in 5′ flanking conserved sequences of wheat low-molecular- weight glutenin subunit genes 1,4* 1,4* 1,4 2 3 LONG Hai , WEI Yuming , YAN Zehong , BAUM Bernard , NEVO Eviatar ZHENG Youliang1,4 1. Triticeae Research Institute, Sichuan Agricultural University, Yaan 625014, China; 2. Agriculture Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, Ottawa K1A 0C6, Canada; 3. Institute of Evolution, University of Haifa, Mt. Carmel, Haifa 31905, Israel; 4. Ministry of Education Key Laboratory of Southwestern Crop Genetic Resources and Improvement, Sichuan Agricultural University, Yaan 611830, China Correspondence should be addressed to Zhen Youliang (email: ylzheng@) Received October 24, 2005; accepted November 30, 2005 Abstract The thirty-three 5′ flanking conserved sequences of the known low-molecular-weight subunit (LMW-GS) genes have been divided into eight clusters, which was in agreement with the classification based on the deduced N-terminal protein sequences. The DNA polymorphism between the eight clusters was obtained by sequence alignment, and a total of 34 polymorphic positions were observed in the approximately 200 bp regions, among which 18 polymorphic positions were candidate SNPs. Seven cluster-specific primer sets were designed for seven out of eight clusters containing cluster-specific bases, with which the genomic DNA of the ditelosomic lines of group 1 chromosomes of a wheat variety ‘Chinese Sprin