《CD5-FC系统对结肠癌细胞的杀伤作用》.pdfVIP

jin，MaOLj 胞L0vo和sw船。的靶向杀伤作用 方法：脂质体法将cEA基因顺式转录调控序列(TRs)驱动 Cheng_】矾Lj，Qjn90¨Ha，Da—NianLai，Jian-GuoLu，xbo-JunWang， Wu，】in—Hao ofGeneral QingWang，Yong—ZhongU，DepartmeⅡt MmtarvMedicm Su曜ery，TaflgduHospital，F0uml Univorslty，Xr孤 组织特异性逆转录病毒载体pcD2分别转导入大肠癌细胞 710038，Sha扭nxiProvince．China， B0-R0ngPan，Depa衄ent()foncolg孔ⅪjmgH08p吼．FounllMjIi忸ry Medic缸Unive娼itv。Ⅺ’蚰710032，Sk且nxi№vince．Ch皿a 5-FC进行敏感试验． o)r惜D0ndence协：Pm￡Bo—R叩gP柚，Depar呲mofonc019y，Ⅺiin2 Hogpital，FourLllMili哪MedicalUIliversi计，Xi’an710032，Sh啪xi Pmvince．china Reoeived：2002—1啦07AcceDbed：2002一ll一25 Abstract AIM：1-0 effectof investigatethe|(i…ng carcinoembryOnic a州gen(匹A)andas轴e-Sped月cc螂ined∞mi响∞(cD)，5- onhumancolo蛐lca嘣noma 月uomcyt|吲ne(5-陀)system ceIlIin郫ⅢvoandSW鹌0加啪吼 vec七or METHoDS：ReCombinantretravi阳l G1CEACDNawas whicht卜皑CD wascontro¨edunderthe constnJ删，in gene f_2 CEA retrdviraIved七0r were promote‘and pCD2 Int∞duced tOthehuman外实验均可观察到明显的旁观者效应． through¨posometechniquerespectively colO陀c七alcarcinomaO剖||ineshn，0and SW480．ExD懈ion was 10wn of(EA and b吐h廿Ie h毡h celIIines惜讲戈t-veIv． ThecelIs cu胁redmG418．1he we吧靶Iec廿vely p巾|ife旧tive 有明显的靶向杀伤效果，但对sw480细胞的杀伤作用小 colOniesweret厂eatedwjth5-FC．