《Kinetic performance comparison of fully and superficially porous particles with sizes ranging between 2.7 lm and 5 lm：Intrinsic evaluation and application to a pharmaceutical test compound》.pdfVIP

Journal of Pharmaceutical Analysis 2013;3(5):313–323 Contents lists available at ScienceDirect Journal of Pharmaceutical Analysis /locate/jpa ORIGINAL ARTICLE Kinetic performance comparison of fully and superficially porous particles with sizes ranging between 2.7 lm and 5 lm: Intrinsic evaluation and application to a pharmaceutical test compound K. Broeckhovena,n, D. Cabooterb, G. Desmeta a Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, 1050 Brussels, Belgium b KU Leuven, Department of Pharmaceutical Sciences, Herestraat 49, 3000 Leuven, Belgium Available online 25 December 2012 KEYWORDS Abstract The reintroduction of superficially porous particles has resulted in a leap forward for the separation performance in liquid chromatography. The underlying reasons for the higher efficiency Kinetic plot; Superficially porous of columns packed with these particles are discussed. The performance of the newly introduced particles; 5 mm superficially porous particles is evaluated and compared to 2.7 mm superficially porous and 5 mm; 3.5 and 5 mm fully porous columns using typical test compounds (alkylphenones) and a relevant Permeability; pharmaceutical compound (impurity of amoxicillin). The 5 mm superficially porous particles Amoxicillin impurity; provide a superior kinetic performance compared to both the 3.5 and 5 mm fully porous particles Alkylphenones over the entire relevant range of separation conditions. The performance of the