柴胡挥发油肝毒性“量—时—毒”关系地研究.pdfVIP

  Research on “Dose - Time -Toxicity ” Relationship of Hepatotoxicity Caused by Volatile Oil from Bupleurum Chinense Speciality:Chinese Materia Medica Author:YANG QianWang Li Tutor:Pro.SUN RongProfessor Sun Rong Abstract Objective:To research and evaluate the “dose-time-toxicity” relationship of hepartotoxicity caused by volatile oil from Bupleurum Chinense in order to provide basis for the elucidation of the hepatotoxical mechanism of volatile oil from Bupleurum Chinense and revelation of scientific connotation of “Bupleurum Chinense robbing hepatic yin”as well as establish foundation for the quality control of Bupleurum Chinense and its preparation,optimization of manufacture technology and the safe application of preparation containing of volatile oil from Bupleurum Chinense . Methods ：The extract technology of volatile oil from Bupleurum Chinense was screened with the guidance of toxicity and its acute toxicity of intragastric administration in rats and mice was researched by the classical acute toxicity test method; the research on “dose-time-toxicity”relationship of acute and chronic hepatotoxicity caused by single or multiple intragastric administration to mice or rats were carried on,and then the level of ALT, AST in serum were tested, and macropathological and pathohistological changes were observed with optical microscope or electron microscope.The hepatotoxical mechanism of volatile oil from Bupleurum Chinense was studied preliminary. Results: The volatile oil from Bupleurum Chinense extracted by the method from Pharmacopeia is optimal to the systematic research of its toxicity.Obvious acute toxicity ca